Lumiprobe Cy5 NHS 酯 Cyanine5 NHS ester

發(fā)表于由
Cy5 NHS 酯 Cyanine5 NHS ester
在過去的一些年，Cy5熒光基因是生命科學(xué)研究和診斷領(lǐng)域非常受歡迎的標(biāo)記試劑。在紅色區(qū)域有最大的熒光發(fā)射，許多CCD檢測器有最大靈敏性，并且背景低。染料顏色非常深，因此即使在凝膠上少至1nmol的量用肉眼也可觀察到。
Cy5 NHS酯是用來標(biāo)記多肽，蛋白和寡核苷酸氨基的活性染料。此染料只需少量的共溶劑（比如DMF或DMSO）來標(biāo)記反應(yīng)。此試劑用來高效標(biāo)記可溶性蛋白，各種多肽和寡核苷酸的氨基。對于較復(fù)雜的靶標(biāo)比如易降解的蛋白，不能用DMF或DMSO時，可以考慮用水溶性sulfo-Cyanine 5 NHS 酯不需要共溶劑，并且兩者有非常相似的熒光特性。
Cy5熒光基因與各種設(shè)備兼容包括許多熒光顯微鏡，成像儀，掃描設(shè)備和熒光成像儀等。
?? Cy5 NHS酯可以替代Alexa Fluor 647, and DyLight 649和Cy3.5?活性酯。
分子結(jié)構(gòu)
一般性質(zhì)
外觀：深藍(lán)色粉末
分子量：616.19
分子式：C36H42ClN3O4
CAS號：1032678-42-4, 350686-88-3
溶解性：易溶于有機溶劑（DMSO,DMF和氯化物），水中的溶解性低。
質(zhì)控：NMR 1H（95%）和13C，TLC, 功能測試
儲存條件：儲存：收到貨后于-20℃避光保存24個月。運輸：室溫3周，避免長時間暴露于陽光，干燥。
光譜性質(zhì)：
最大激發(fā)波長：646
在最大激發(fā)波長下的消光系數(shù)：250000
最大發(fā)射波長：662
熒光量子產(chǎn)率：0.2

During the last years, Cyanine5 (an analog of Cy5?) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1?nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5? NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

General properties

Appearance: dark blue powder
Molecular weight: 616.19
CAS number: 1032678-42-4, 350686-88-3
Molecular formula: C36H42ClN3O4
Solubility: good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents, low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Cat. # Quantity Price Lead time
13020 1 mg 110.00$ in stock
23020 5 mg 210.00$ in stock
43020 25 mg 410.00$ in stock
53020 50 mg 695.00$ in stock
63020 100 mg 1190.00$ in stock
Product citations ?參考文獻
  1. Li, L.; Sun, W.; Li, L.; Liu, Y.; Wu, L.; Wang, F.; Zhou, Z.; Zhang, Z.; Huang, Y. A pH-responsive sequential-disassembly nanohybrid for mitochondrial targeting. Nanoscale, in press. doi: 10.1039/c6nr07004c
  2. Matsuo, Y.; Maurer, S.P.; Yukawa, M.; Zakian, S.; Singleton, M.R.; Surrey, T.; Toda, T. An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation. Journal of Cell Science. doi: 10.1242/jcs.197533
  3. Stephen, Z.R.; Gebhart, R.N.; Jeon, M.; Blair, A.A.; Ellenbogen, R.G.; Silber, J.R.; Zhang, M. pH-Sensitive O6-Benzylguanosine Polymer Modified Magnetic Nanoparticles for Treatment of Glioblastomas. Bioconjugate Chemistry, in press. doi:10.1021/acs.bioconjchem.6b00545
  4. Bloch, M.B.D.; Yavin, E.; Nissan, A.; Ariel, I.; Kenett, R.; Brass, D.; Rubinstein, A. The effect of linker type and recognition peptide conjugation chemistry on tissue affinity and cytotoxicity of charged polyacrylamide. Journal of Controlled Release, in press. doi:10.1016/j.jconrel.2016.06.038