Lumiprobe BDP FL NHS ester
品牌：Lumiprobe
官方代理商：上海金畔生物科技有限公司（有授權(quán)證書(shū)）
貨期：僅需要5-7工作日，不需要像其他家攢單導(dǎo)致貨期3-4周。
Lumiprobe BDP FL NHS ester
| Cat. # | Quantity | Lead time |
| 11420 | 1 mg | in stock |
| 21420 | 5 mg | in stock |
| 41420 | 25 mg | in stock |
| 51420 | 50 mg | in stock |
| 61420 | 100 mg | in stock |
BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein, a molecule identical to BODIPY FL? NHS ester. An amino-reactive dye for the labeling of proteins and peptides.
While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BODIPY-FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.
The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.
The dye is a good replacement for fluorescein (FAM), BODIPY-FL, Alexa Fluor 488, DyLight 488, Cy2, and other 488 nm dyes.
Absorption and emission spectra of BDP FL
General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 274.1 |
| Molecular weight: | 389.16 |
| CAS number: | 146616-66-2 |
| Molecular formula: | C18H18BF2N3O4 |
| Solubility: | Good in organic solvents (DMF, DMSO, dichlromethane), limited in water |
| Quality control: | NMR 1H, HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 503 |
| ε, L?mol?1?cm?1: | 80000 |
| Emission maximum, nm: | 509 |
| Fluorescence quantum yield: | 0.97 |
| CF260: | 0.015 |
| CF280: | 0.027 |
Product citations
- Poreba, M.; Rut, W.; Vizovisek, M.; Groborz, K.; Kasperkiewicz, P.; Finlay, D.; Vuori, K.; Turk, D.; Turk, B.; Salvesen, G.; Drag, M.Selective imaging of human cathepsin L in breast cancer by fluorescent activity-based probes. Chemical Science, 2018, 9(8), 2113–2129. doi: 10.1039/C7SC04303A
- Grube, L.; Dellen, R.; Kruse, F.; Schwender, H.; Stuehler, K.; Poschmann, G. Mining the secretome of C2C12 muscle cells: Data dependent experimental approach to analyze protein secretion using label-free quantification and peptide based analysis. Journal of Proteome Research, 2018, 17(2), 879–890. doi: 10.1021/acs.jproteome.7b00684
- Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
- Gaspar, I.; Wippich, F.; Ephrussi, A. Enzymatic production of single molecule FISH and RNA capture probes. RNA, 2017, 23(10), 1582–1591. doi: 10.1261/rna.061184.117
Bodipy(BDP)氟硼熒光活性染料發(fā)展歷史
Bodipy二吡咯類熒光染料相對(duì)比于熒光素、羅丹明或者菁染料有著較高的選擇性、高靈敏度、高熒光量子產(chǎn)率、高摩爾消光系數(shù)、良好的光學(xué)穩(wěn)定性和對(duì)PH不敏感的優(yōu)點(diǎn)被廣泛應(yīng)用于生物標(biāo)記、熒光探針及生物成像，西安瑞禧生物科技有限公司可以提供種類豐富的Bodipy熒光染料，熒光波長(zhǎng)可定制 從550-800之間，末端可以修飾各種活性基團(tuán)。
Bodipy類熒光染料具有良好的穩(wěn)定性、優(yōu)良的光學(xué)性能，主要表現(xiàn)在以下幾個(gè)方面：
1：BODIPY的光譜峰寬比較窄，在光學(xué)領(lǐng)域和生物學(xué)領(lǐng)域進(jìn)行分析檢測(cè)時(shí)，光譜峰寬較窄，檢測(cè)靈敏度較高，并且減少了待測(cè)樣品的用量。測(cè)得的結(jié)果中，熒光光譜上會(huì)出現(xiàn)更為明顯的熒光吸收和發(fā)射的變化，這樣更精確地測(cè)得了樣品的含量。
2：在整個(gè)BODIPY分子中，并不存在負(fù)電荷。因而在溶液中，溶液中的離子無(wú)法與缺少電荷的BODIPY分子發(fā)生靜電作用，這樣非常有效的避免了測(cè)定時(shí)靜電作用對(duì)測(cè)量結(jié)果的干擾，使測(cè)定過(guò)程更加穩(wěn)定，測(cè)得結(jié)果更加精準(zhǔn)。
3：BODIPY類熒光染料的光穩(wěn)定性能非常優(yōu)越，當(dāng)它被激發(fā)到達(dá)激發(fā)態(tài)時(shí)，不會(huì)因?yàn)楹荛L(zhǎng)時(shí)間的激發(fā)而在分析測(cè)定過(guò)程中發(fā)生激發(fā)態(tài)的分解或其他變化，這樣測(cè)得的熒光光譜更加準(zhǔn)確可信。
4：BODIPY染料，其熒光量子產(chǎn)率甚至接近1.0。一般普通的染料在水溶液中其熒光量子產(chǎn)率會(huì)變低，有的甚至被猝滅。對(duì)于BODIPY分子來(lái)說(shuō)，在水溶液中其熒光量子產(chǎn)率雖然有所下降，但它仍然能保持較高的熒光量子產(chǎn)率。
5：由BODIPY的母體結(jié)構(gòu)可知，他的母體結(jié)構(gòu)上的取代基一般不會(huì)出現(xiàn)氨基或羧基這類對(duì)酸堿敏感的活性基團(tuán)。因此外界的酸堿度對(duì)其影響會(huì)大幅度減小，因此BODIPY染料不會(huì)輕易變質(zhì)。
6：BODIPY類熒光染料對(duì)光的感應(yīng)度很高，并且它還有高的摩爾消光系數(shù)，有些經(jīng)過(guò)修飾后的BODIPY染料在水溶液中的溶解度也較高，再加上它在水中不易被猝滅這一優(yōu)異的性能，使得BODIPY在光學(xué)、生物學(xué)、分析學(xué)領(lǐng)域有著極其廣泛的應(yīng)用。
西安瑞禧生物科技有限公司可以提供激發(fā)和發(fā)射波長(zhǎng)從500nm-800nm的活性染料（包括800nm左右的近紅外）并且可以修飾NHS、MAL、NH2、COOH、N3、SH等不同活性基團(tuán)。
常規(guī)的Bodipy熒光染料都是激發(fā)發(fā)射波長(zhǎng)都是在500-650nm之間的，包括國(guó)外的商家也只能提供500-650nm波長(zhǎng)的熒光染料，瑞禧生物不僅可以提供波長(zhǎng)在500-600nm之間的波長(zhǎng) 還可以提供650-800nm之間的近紅外波段的BDP
500-800之間的波長(zhǎng)可以選擇：
Bodipy carboxylic acid（羧基COOH）
Bodipy NHS ester（活化脂NHS）
Bodipy amine （NH2氨基）
Bodipy maleimide （MAL馬來(lái)酰亞胺）
Bodipy azide （N3疊氮）
Bodipy alkyne （ALK炔烴）
Bodipy hydrazide （HZ酰基）
Bodipy Thiol （HS巰基）
產(chǎn)品列表：
BODIPY™ FL NHS Ester (Succinimidyl Ester)
BODIPY™ FL Sulfonated Succinimidyl Ester
BODIPY™ FL-X NHS Ester (Succinimidyl Ester)
BODIPY™ TMR-X NHS Ester (Succinimidyl Ester)
BODIPY™ 530/550 NHS Ester (Succinimidyl Ester)
BODIPY™ R6G NHS Ester (Succinimidyl Ester)
BODIPY™ 581/591 NHS Ester (Succinimidyl Ester)
BODIPY™ 576/589 NHS Ester (Succinimidyl Ester)
BODIPY™ 650/665-X NHS Ester (Succinimidyl Ester)
BODIPY™ 493/503 NHS Ester (Succinimidyl Ester)
BODIPY™ 558/568 NHS Ester (Succinimidyl Ester)
BODIPY™ TR-X NHS Ester (Succinimidyl Ester)
BODIPY™ 630/650-X NHS Ester (Succinimidyl Ester)
BODIPY™ FL maleimide
BODIPY™ FL azide
BODIPY™ FL alkyne
BODIPY™ FL hydrazide
BODIPY™ FL amine
BODIPY™ FL Carboxylic acid
BODIPY™ TR ceramide
BODIPY™ FL STP ester
BODIPY™ FL C12-sphingomyelin
BODIPY™ R6G NHS Ester
BODIPY™ 558/568 phalloidin
BODIPY™ FL hydrazide
BODIPY™ TMR-X NHS Ester
BODIPY™ FL L-cystine
BODIPY™ FL Carboxylic acid
BODIPY™ FL Carboxylic acid
BODIPY™ FL Carboxylic acid
BODIPY?493/503 NHS ester
BODIPY?493/503 Carboxylic acid
BODIPY?493/503 amine
BODIPY?493/503 maleimide
BODIPY?493/503 azide
BODIPY?493/503 alkyne
BODIPY?558/568 NHS ester
BODIPY?558/568 Carboxylic acid
BODIPY?558/568 amine
BODIPY?558/568 maleimide
BODIPY?558/568 azide
BODIPY?558/568 alkyne
BODIPY?650/665 NHS ester
BODIPY?650/665 Carboxylic acid
BODIPY?650/665 amine
BODIPY?650/665 maleimide
BODIPY?650/665 azide
BODIPY?650/665 alkyne
BODIPY?FL NHS Ester
BODIPY?TMR NHS Ester
BODIPY?TR NHS Ester
BODIPY?FL amine
BODIPY?TMR amine
BODIPY?TR amine
?
以下染料的激發(fā)波長(zhǎng)是685nm 發(fā)射波長(zhǎng)是720nm
Azo-Bodipy-685? (ABDP685)
Azo-Bodipy 685 carboxylic acid
Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 carboxylic acid
Azo-Bodipy 685 NHS ester
Sulfo-Azo-Bodipy 685 NHS ester
Azo-Bodipy 685 amine
Sulfo-Azo-Bodipy 685 amine
PEG-Azo-Bodipy 685 NHS ester
PEG- Azo-Bodipy 685 amine
Azo-Bodipy 685 alkyne
Sulfo-Azo-Bodipy 685 alkyne
Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 azide
Azo-Bodipy 685 maleimide
Sulfo-Azo-Bodipy 685 maleimide
Azo-Bodipy 685 pH6.5
我們還接受激發(fā)波長(zhǎng)和發(fā)射波長(zhǎng)在500nm-800nm之間的BDP活性熒光染料的定制產(chǎn)品。
上面所述染料可以連接：COOH,? NH2,? NHS,? MAL,? N3,? ALK,? hydrazide,? SH
其他波長(zhǎng)的BDP活性染料的產(chǎn)品列表：
產(chǎn)品列表：
BDP 558/568 NHS ester
BDP 581/591 NHS ester
BDP 630/650 X NHS ester
BDP FL NHS ester
BDP R6G NHS ester
BDP TMR NHS ester
BDP TR NHS ester
BDP 581/591 azide
BDP 630/650 azide
BDP FL azide
BDP R6G azide
BDP TMR azide
BDP TR azide
BDP 558/568 alkyne
BDP 581/591 alkyne
BDP 630/650 alkyne
BDP FL alkyne
BDP R6G alkyne
BDP TMR alkyne
BDP TR alkyne
BDP 581/591 maleimide
BDP 630/650 maleimide
BDP FL maleimide
BDP R6G maleimide
BDP TMR maleimide
BDP TR maleimide
BDP 581/591 hydrazide
BDP 630/650 hydrazide
BDP FL hydrazide
BDP TR hydrazide
BDP 558/568 carboxylic acid
BDP 581/591 carboxylic acid
BDP 630/650 carboxylic acid
BDP FL carboxylic acid
BDP R6G carboxylic acid
BDP TMR carboxylic acid
BDP TR carboxylic acid
BDP 581/591 amine
BDP 630/650 amine
BDP FL amine
BDP R6G amine
BDP TMR amine
BDP TR amine
BDP 581/591 tetrazine
BDP 630/650 tetrazine
BDP FL tetrazine
BDP TR tetrazine
BODIPY母體結(jié)構(gòu)最初是由德國(guó)化學(xué)家Treibs, A.和kreuzer, F. H.在1968年合成的，他們合成的BODIPY 類熒光染料 [40] 。另外，他們還合成了BODIPY染料的母體結(jié)構(gòu)。在BODIPY母體結(jié)構(gòu)中，Treibs, A.和kreuzer, F. H.在R1-R7中用不同基團(tuán)取代得到不同的衍生產(chǎn)物。人們根據(jù)Treibs, A.和kreuzer, F. H.的前期探索，對(duì)BODIPY類染料做出了多種結(jié)構(gòu)修飾，這大大豐富了BODIPY的種類，使其迅猛發(fā)展。