Sigma 孕酮Progesterone

產(chǎn)品編號 P8783 | CAS號 57-83-0 | SIGMA

英文名:Progesterone
中文名:1.孕甾酮 2.黃體酮 3.黃體素?4.孕酮? 5.孕醇 6.黃體激素
Synonym: 4-Pregnene-3,20-dione
CAS Number 57-83-0
Empirical Formula (Hill Notation) C21H30O2
Molecular Weight 314.46
description:synthesized from materials derived from plant source
product line:BioReagent
form:powder
potency:0.1-20 ng/mL
mp:128-132 °C(lit.)
solubility :absolute ethanol: soluble1 mg/mL
suitability :uitable for cell culture

Application

用于細(xì)胞培養(yǎng)，研究細(xì)胞信號傳導(dǎo)和基因表達(dá)的甾醇調(diào)控。

Other Notes

由卵巢黃體產(chǎn)生的類固醇激素。

包裝：1, 5, 25 g in glass bottle

Reconstitution

To prepare 20μg/ml stock solution, add 1ml absolute ethanol per mg progesterone, gently swirl to dissolve, add 49ml sterile medium per ml ethanol.

Physical form

室溫下為粉末狀；分成小份凍存，以避免使用時反復(fù)冷凍/解凍

Biochem/physiol Actions

誘導(dǎo)子宮內(nèi)皮細(xì)胞的成熟和分泌活動;抑制排卵。孕酮與乳腺癌的病因有關(guān)。

Sigma人血管緊張素 II

產(chǎn)品編號 A9525 | CAS號 4474-91-3 | SIGMA

Angiotensin II human ? ?≥93% (HPLC), powder

Sigma絲裂霉素 C 來源于頭狀鏈霉菌

產(chǎn)品編號 M0503-2mg?| CAS號 50-07-7 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

DNA 合成、細(xì)胞核分裂和癌細(xì)胞的抑制劑。對革蘭氏陽性菌、革蘭氏陰性菌、抗酸桿菌有抗菌性。

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared – the precipitated solution has been proven toxic to cells.

Preparation Note

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 阿霉素 鹽酸鹽

產(chǎn)品編號 D1515 | CAS號 25316-40-9 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

包裝:10 mg in glass bottle?Biochem/physiol Actions

Naturally fluorescent anthracycline antibiotic, anticancer drug. Doxorubicin is a substrate of MRP1 which was first cloned from a DOX-resistant lung cancer cell line. Fluorescent property has been exploited for the measurement of drug efflux pump activities as well as resolving the important question of intracellular localization of various multidrug resistance proteins and the role of subcellular organelles (Golgi and lysosome) in the sequestration of drugs and its implication in drug resistant phenotypes.

法律信息

Adriamycin 為以下機(jī)構(gòu)的注冊商標(biāo)： RDF Pharmacia & Upjohn S.P.A.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 放線菌素 D

產(chǎn)品編號 A9415 | CAS號 50-76-0 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

包裝：2, 5, 10, 25 mg in glass bottle

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 二甲基亞砜DMSO

產(chǎn)品編號 D2650 | CAS號 67-68-5 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DMSO 是一種用于化學(xué)反應(yīng)（如聚合酶鏈?zhǔn)椒磻?yīng) (PCR)）的極性非質(zhì)子溶劑，也可作為冷凍保護(hù)玻璃化劑用于保存細(xì)胞、組織和器官。DMSO 可在細(xì)胞冷凍培養(yǎng)基中用于保護(hù)細(xì)胞免受冰晶導(dǎo)致的機(jī)械損傷。它可用于原代、繼代培養(yǎng)和重組的異倍體和雜交瘤細(xì)胞系、胚胎干細(xì)胞 (ESC) 以及造血干細(xì)胞的凍存。DMSO 常與 BSA 或胎牛血清 (FBS) 一同使用。

Caution

容易過度冷卻，并在室溫下緩慢融化固化的產(chǎn)品放在室溫下可以再次液化，對其本身無影響。

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

包裝:5mL 和 10mL 火焰封口安瓿瓶包裝，100mL 棕色瓶包裝

sigma 醋氨酚BioXtra

產(chǎn)品編號 A7085 | CAS號 103-90-2 | SIGMA-ALDRICH

Application：鎮(zhèn)痛劑。

包裝：1 kg in glass bottle，100, 500 g in glass bottle

Sigma特殊產(chǎn)品Thiopropyl–Sepharose? 6B

產(chǎn)品編號 T8387 | CAS號 68517-67-9 | SIGMA

包裝：1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Thiopropyl Sepharose^? 6B is used in protein chromatography, affinity chromatography and hydrophobic interactions. Thiopropyl Sepharose^? 6B has been used for the removal of inhibitors from PCR extracts from evidence collected for law enforcement murder investigations. Thiopropyl Sepharose^? 6B has also been used to describe a method for the investigation of functional properties of distinct domains of viral thiol proteins, including the influenza virus membrane M1 protein.

法律信息

Sepharose is a registered trademark of GE Healthcare

sigma-白蛋白 來源于牛血清

產(chǎn)品編號 A3803 | CAS號 9048-46-8 | SIGMA

包裝：10, 50, 100 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn^[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

采用熱休克分級分離法制備。經(jīng)過進(jìn)一步純化以降低脂肪酸含量。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

產(chǎn)品編號 A8806 | CAS號 9048-46-8 | SIGMA

包裝：1, 5 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn^[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

通常稱為 Cohn fraction V；本品采用改進(jìn)的 Cohn 氏冷乙醇分級分離法制備。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.^[]

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide