TSKgel G40000PWXL 凝膠過濾色譜柱

TSKgel G40000PWXL 凝膠過濾色譜柱

TSK凝膠色譜柱G3000SWXL的填料是親水而剛性,多孔球型,化學(xué)和機(jī)械性能都非常優(yōu)異的聚合物。適用于以凝膠色譜的模式分析分離蛋白質(zhì)、多肽、多糖、寡糖、DNA、RNA、水溶性的有機(jī)聚合物和其他水溶性大分子樣品等.
PW系列凝膠色譜柱的填料是親水而剛性,多孔球型,化學(xué)和機(jī)械性能都非常優(yōu)異的聚合物。適用于以凝膠色譜的模式分析分離蛋白質(zhì)、多肽、多糖、寡糖、DNA、RNA、水溶性的有機(jī)聚合物和其他水溶性大分子樣品等。PW系列色譜柱的PH適用范圍為2-12,可使用含有50%的混合有機(jī)溶劑為流動相,使用溫度可達(dá)80℃(TSK-gel?G-DNA-PW為50℃)。盡管大多數(shù)的PW填料呈微弱的負(fù)電性(約5~8μ?eq/mL),但是對于分析分離非離子、陰離子和大多數(shù)的陽離子型樣品沒有任何影響。因為PW填料具有非常寬的分離范圍,更加線性的校正曲線以及比SW色譜柱更加低得多的吸附特性,所PW色譜柱經(jīng)常用于分離水溶性的合成聚合物。
相對而言,SW色譜柱適合于分析分離單分散性的生物大分子,如蛋白等,而PW色譜柱則更加適合于如多糖和合成聚合物等多分散性樣品的分析分離。
TSK凝膠色譜柱特點:
1.?TSK-GEL凝膠柱具有強(qiáng)大的適用性和可靠性,其主要產(chǎn)品有SW、PW等系列
2.?SWXL?及PWXL相對SW和PW而言,具有更好的峰分辨率;而SW和PW柱具有較大
樣品的承載能力
3.?TSK-GEL?SW色譜柱適合于分析單分散性的生物大分子,如蛋白、肽等,
TSK-GEL?PW色譜柱更加適合于如多糖和合成聚合物等多分散性的分析分離
TSK-GEL SW系列部分色譜柱填料的種類及特性

TSKgel G4000PWxl尺寸排阻色譜柱

產(chǎn)品特點

  1. TSKgel PWxl柱由親水性球形聚甲基丙烯酸酯顆粒填充。
  2. 使用柱效更高的PWxl型柱去實現(xiàn)最佳分辨率,從而縮短分析時間,或在樣品量受限的情況中分析樣品。
  3. pH值穩(wěn)定在2~12以內(nèi)。
  4. 淋洗液:水或緩沖劑(最高可達(dá)20%甲醇/80%水)。
  5. 溫度可達(dá)80℃(TSKgel G-DNA-PW的溫度為50℃)。
  6. 粒度較小的PWxl型樹脂具有更高的交聯(lián)度。
基質(zhì) 聚甲基丙烯酸酯
粒徑 10μm
孔徑 450A
pH?范圍 2~12
Max?有機(jī)相% 20%
排阻限 300000Da(聚乙二醇)

訂貨信息:

貨號 描述
008022 TSKgel G4000PWxl,7μm,7.8mmI.D.×30cm
008033 Guard Column for ? P/N 08022,12μm,6.0mmI.D.×4cm
008035 TSKgel Top-Off for ? PWxl, 1g,10μm
品牌 粒度(μm) 孔徑(?) 樣品的分子量
球蛋白 葡聚糖 聚乙二醇
G2000SWXL 5 125 5,000~150,000 1,000~30,000 500~15,000
G2000SW 10 125 5,000~100,000 1,000~30,000 500~15,000
G3000SWXL 5 250 10,000~500,000 2,000~70,000 1,000~35,000
G3000SW 10 250 10,000~500,000 2,000~70,000 1,000~35,000
G4000SWXL 8 450 20,000~10,000,000 4,000~500,000 2,000~250,000
G4000SW 17 450 20,000~7,000,000 4,000~500,000 2,000~250,000

TSK-GEL PW系列色譜柱部分填料的種類及特性

品牌 粒度(μm) 孔徑(?) 樣品的分子量
球蛋白 葡聚糖 聚乙二醇
G2000PWXL 10,17,20 125 <5,000 可達(dá)2,000
G3000PWXL 6 200 500~800,000 可達(dá)60,000 可達(dá)50,000
G3000PW 10,17,20 ~ ~ ~ ~
G4000PWXL 10 500 10,000~1,500,000 1,000~700,000 2,000~300,000
G4000PW 17,22 ~ ~ ~ ~
G5000PWXL 10 1000 <10,000,000 50,000~7,000,000 4,000~1,000,000
G5000PW 17,20,22 ~ ~ ~ ~
G6000PWXL 13 >1000 <200,000,000 500,000-50,000,000 4,000~8,000,000
G6000PW 17,25 ~ ~ ~ ~

訂貨資料

訂貨號 品牌 內(nèi)徑(mm) ?長度(cm) 粒度
(μm)
理論塔板數(shù) 流速(mL/min) 最大壓力(kgf/cm2
范圍 最大
08540

08541

08542

G2000SWXL

G3000SWXL
G4000SWXL

7.8
7.8
7.8
30
30
30
5
5
20,000
20,000
16,000
0.5-1.0
0.5-1.0
0.5-1.0
1.2
1.2
1.2
70
70
35
05788

05789

05790

G2000SW

G3000SW

G4000SW

7.5
7.5
7.5
30
30
30
8
10
10
10,000
10,000
8,000
0.5-1.0
0.5-1.0
0.5-1.0
1.2
1.2
1.2
20
25
15
05102

05103

05104

G2000SW

G3000SW

G4000SW

7.5
7.5
7.5
60
60
60
13
10
10
20,000
20,000
16,000
0.5-1.0
0.5-1.0
0.5-1.0
1.2
1.2
1.2
40
50
30
08021

08022

08023

08024

G3000PWXL
G4000PWXL

G5000PWXL
G6000PWXL

7.8
7.8
7.8
7.8
30
30
30
30
13
6
10
10
14,000
10,000
10,000
7,000
0.5-0.8
0.3-0.8
0.3-0.8
0.3-0.8
1.0
1.0
1.0
1.0
40
20
20
20

日本TOSOH 分析色譜柱-TOSOH代理商

日本TOSOH 分析色譜柱-TOSOH代理商

日本東曹株式會社(TOSOH CORPORATION)始建于1935年,原名東洋曹達(dá)工業(yè)株式會社,現(xiàn)已發(fā)展成為涉足石油化工、基礎(chǔ)化學(xué)、精細(xì)化工、電子材料、生命科學(xué)等多項事業(yè)的全球性跨國企業(yè),2009年度全球銷售額已超過80億美金。
主要業(yè)務(wù)集中在化工原料、精細(xì)化工產(chǎn)品、電子材料及色譜層析產(chǎn)品的銷售上。

Size-exclusion chromatography (SEC)
SEC separates molecules based on their size, or more
precisely, their hydrodynamic volume. It is a well known
technique for the separation of macromolecules like natural
and synthetic polymers. When an aqueous eluent is used,
SEC is also referred to as gel filtration chromatography
(GFC). When an organic eluent is used, SEC is referred to as
gel permeation chromatography (GPC). GPC is the method
of choice for the characterization of polymer molecular
weight and molecular weight distribution.
Tosoh Bioscience is a leading manufacturer of SEC/GPC
columns and developed GPC instruments for the Asian
market for more than 35 years. This brochure gives an
overview over the Tosoh Bioscience GPC/SEC product
portfolio for the separation of water and organic soluble
polymers. The separation of proteins and biopolymers with
silica based TSKgel SEC columns is not covered here.

Gel Permeation Chromatography (GPC)
GPC plays an important role in the characterization of
organic-soluble polymers in the chemical and petrochemical
industries. TSKgel GPC columns contain particles prepared
from polystyrene crosslinked with divinylbenzene. Available
products are grouped according to their relative lack of
adsorptive properties and the speed of analysis.

貨號 品名及描述 產(chǎn)品分類
5102 G2000SW?7.5*600 Column
5103 G3000SW?7.5*600 Column
5104 G4000SW?7.5*600 Column
5105 G2000PW?7.5*600 Column
5106 G3000PW?7.5*600 Column
5107 G4000PW?7.5*600 Column
5108 G5000PW?7.5*600 Column
5109 G6000PW?7.5*600 Column
5146 G2000SW?21.5*600 Column
5147 G3000SW?21.5*600 Column
5148 G4000SW?21.5*600 Column
5202 STD?PSA-300(11587) Std.?Polymer
5203 STD?PS?A-500(11589) Std.?Polymer
5204 STD?PS?A-1000(11591) Std.?Polymer
5205 STD?PS?A-2500(11593) Std.?Polymer
5206 STD?PS?A-5000(11595) Std.?Polymer
5207 STD?PS?F-1(11597) Std.?Polymer
5208 STD?PS?F-2(11599) Std.?Polymer
5209 STD?PS?F-4(11601) Std.?Polymer
5210 STD?PS?F-10(11603) Std.?Polymer
5211 STD?PS?F-20 Std.?Polymer
5212 STD?PS?F-40(11607) Std.?Polymer
5213 STD?PS?F-80 Std.?Polymer
5214 STD?PS?F-128(11611) Std.?Polymer
5215 STD?PS?F-288(11613) Std.?Polymer
5216 STD?PS?F-380 Std.?Polymer
5217 STD?PS?F-450 Std.?Polymer
5218 STD?PS?F-550 Std.?Polymer
5219 STD?PS?F-700 Std.?Polymer
5220 STD?PS?F-850 Std.?Polymer
5221 STD?PS?F-2000 Std.?Polymer
5371 Guard?SW?7.5*75 Column
5748 ENDFITTING3/81/1610F” parts
5758 Guard?SW?21.5*75 Column
5760 G1000PW?7.5*300 Column
5761 G2000PW?7.5*300 Column
5762 G3000PW?7.5*300 Column
5763 G4000PW?7.5*300 Column
5764 G5000PW?7.5*300 Column
5765 G6000PW?7.5*300 Column
5773 STD?O?SE-KIT Std.?Polymer
5788 G2000SW?7.5*300 Column
5789 G3000SW?7.5*300 Column
5790 G4000SW?7.5*300 Column
6172 ODS-120A?21.5*300 Column
6191 ENDFITTING1RU1/1610F” parts
6211 STD?O?SE-2(11573) Std.?Polymer
6212 STD?O?SE-5(11575) Std.?Polymer
6213 STD?O?SE-8(11577) Std.?Polymer
6214 STD?O?SE-15(11579) Std.?Polymer
6215 STD?O?SE-30(11581) Std.?Polymer
6216 STD?O?SE-70(11583) Std.?Polymer
6217 STD?O?SE-150(11585) Std.?Polymer
6476 STD?PS?Oligomer?Kit Std.?Polymer
6477 STD?PS?High?Polymer?Kit Std.?Polymer
6727 G2000SW?21.5*300 Column
6728 G3000SW?21.5*300 Column
6729 G4000SW?21.5*300 Column
6758 Guard?PWH?21.5*75 Column
6762 Guard?PWH?7.5*75 Column
6763 Guard?PWL?7.5*75 Column
6819 TOPOFF?SW,?1mL Bulk?gel
6837 IC?Anion-PW,?4.6*50 ?Column
6839 IC-Anion-SW?4.6*50 Column
7113 Guard?HXL?6.0*40 Column
7124 ODS-120A?4.6*250 Column
7125 ODS-120T?4.6*250 Column
7127 NH2-60?4.6*250 Column
7128 OH-120,?4.6*250m ?Column
7129 ODS-120A?7.8*300 Column
7130 ODS-120T?7.8*300 Column
7134 ODS-120T?21.5*300 Column
7147 Silica-60,?4.6*250 ?Column
7156 SCX?6.0*150 Column
7157 SAX?6.0*150 Column
7158 SCX?7.8*300 Column
7161 SP-5PW?7.5*75 Column
7162 CM-3SW?7.5*75 Column
7163 DEAE-3SW?7.5*75 Column
7164 DEAE-5PW?7.5*75 Column
7165 SP-2SW?4.6*250 Column
7166 QAE-2SW?4.6*250 Column
7167 CM-2SW?4.6*250 Column
7168 DEAE-2SW?4.6*250 Column
7190 TMS-250?4.6*75 Column
7210 Guard?DEAE-5PW?Kit Column
7211 Guardkit?SP-5PW Column
7447 HW-40S?500mL Toyoarl
7448 HW-40F?500mL Toyoarl
7449 HW-40C?500mL Toyoarl
7450 HW-40EC?500mL Toyoarl
7451 HW-40S?250mL Toyoarl
7452 HW-50S?500mL Toyoarl
7453 HW-50F?500mL Toyoarl
7455 HW-50S?250mL Toyoarl
7456 HW-55S?500mL Toyoarl