SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD Protein (His&AVI Tag)，Biotinylated
純度
> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
內(nèi)毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物素標記率
0.7-1 as determined by the HABA assay.
生物活性
Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2(BA.4/BA.5/BA.5.2)Spike RBD Protein(His&AVI Tag),Biotinylated , the EC50 is 15-45 ng/mL.
蛋白構(gòu)建
A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.4/BA.5/BA.5.2).
表達宿主
HEK293 Cells
種屬
SARS-CoV-2
預測 N 端
Arg 319
分子量
The recombinant SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD consists of 249 amino acids and predicts a molecular mass of 28.49 kDa. As a result of glycosylation, it migrates as an approximately 38.44 kDa band in SDS-PAGE under reducing conditions.
緩沖液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
運輸方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
穩(wěn)定性 & 儲存條件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
復溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD Protein (His&AVI Tag)，Biotinylated 圖片
