Lucigen | LGC代理,sbeadex Tissue DNA Purification Kit,部分現(xiàn)貨


sbeadex Tissue DNA Purification Kit

The sbeadex Tissue DNA Purification Kit uses magnetic bead technology to purify high-quality genomic DNA from a range of human and animal tissues and cultured cells.

Product Details

The sbeadex Tissue DNA Purification Kit contains two wash buffers, a lysis buffer, binding buffer, a binding additive, an elution buffer and sbeadex particle suspension.

Compatible tissue types: mouse tails, mouse lung, ear punches, skin, spleen, muscle 

Choose between 96 or 960 preparations, or enquire for bulk options. 

Key features

  • Efficient purification of high-quality DNA from diverse mammalian tissues and cultured cells in just one hour
  • Streamline your workflow with ready-to-use buffers
  • Easy scale up sample processing using popular robotic platforms like Hamilton, TECAN, or KingFisher™, saving time and effort
  • Ensure accurate downstream applications with final wash buffer that is PCR inhibitor free and does not contain organic solvents or chaotropic salts
  • Customize your protocol to your specific needs by adjusting batch size, lysis steps, and elution steps

Select a Number of purifications

Please select a Number of purifications

Lucigen | LGC, Biosearch Technologies主要開發(fā)各類用于基因克隆的試劑盒及相關(guān)產(chǎn)品,包括:CloneSmart?平端克隆試劑盒、BigEasy?線性克隆系統(tǒng)、pEZSeq™平端克隆試劑盒、聚合酶及E.cloni?感受態(tài)細(xì)胞及蛋白表達(dá)系統(tǒng)等。

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bioGenous腫瘤組織消化液 Tumor Tissue Digestion Solution(K601003)

Tumor Tissue Digestion Solution 腫瘤組織消化液

貨號(hào):K601003

規(guī)格:100ml

500ml

品牌:bioGenous

產(chǎn)品介紹

bioGenousTM Tumor Tissue Digestion Solution(腫瘤組織消化液)可將腫瘤組織樣本溫和、快速地消化解離為細(xì)胞懸液或細(xì)胞團(tuán)塊,可用于后續(xù)腫瘤類器官的構(gòu)建。該組織消化液廣泛適用于實(shí)體腫瘤(如腸癌、肺癌、乳腺癌、子宮內(nèi)膜癌等)及正常組織標(biāo)本在體外原代培養(yǎng)的消化解離。

產(chǎn)品信息

產(chǎn)品組成

貨號(hào)

規(guī)格

儲(chǔ)存條件及周期

bioGenousTMTumor Tissue Digestion Solution(Basal Medium)

K601003-A-100/A500

100mL/500mL

4℃,12 個(gè)月

bioGenousTM Tumor Tissue Digestion Solution(Supplement 20X)

K601003-B-100/A500

5mL/25mL

-20℃,12個(gè)月

腫瘤組織消化過程中可能應(yīng)用的其他試劑

試劑

貨號(hào)

Cancer Organoid Basal Medium

B213152

Fetal Bovine Serum, FBS

腫瘤組織消化液的配制

無菌條件下配制腫瘤組織消化液,下面是配制10mL完全腫瘤組織消化液的示例,如果需要制備其他體積,可自行相應(yīng)調(diào)整。

 

1.         4℃解凍bioGenousTMTumor Tissue Digestion Solution(Supplement 20X),解凍后充分混勻。

注意:解凍后,建議將bioGenousTM Tumor Tissue Digestion Solution (Supplement 20X) 分裝后保存,按需取用,避免多次反復(fù)凍融。

2.         500ubioGenousTM Tumor Tissue Digestion Solution(Supplement 20X)加至9.5mLbioGenousTMTumor Tissue Digestion Solution(Basal Medium) 中,充分混合,配制成10mL腫瘤組織消化液。

注意:配制后的完全腫瘤組織消化液可在2-8°C儲(chǔ)存,建議24小時(shí)內(nèi)使用,或-20℃儲(chǔ)存1。

腫瘤組織樣本的消化

1.  在消化之前利用手術(shù)剪或手術(shù)刀將腫瘤組織剪切成體積約為1~3 mm3的碎片。

2.  根據(jù)原組織塊大小加入適當(dāng)體積的(消化液體積需是原腫瘤組織體積的25-50倍)Tumor Tissue Digestion Solution(腫瘤組織消化液)后置于37℃恒溫培養(yǎng)箱或恒溫?fù)u床中進(jìn)行組織消化。不同種類腫瘤的樣本,因其組織來源、腫瘤亞型及個(gè)體化差異,其消化所需時(shí)間可能會(huì)有所不同,總體消化時(shí)間在30min-120min。

注意:在此操作過程中須仔細(xì)監(jiān)測(cè)消化過程,因?yàn)檫^度消化可能會(huì)顯著降低類器官形成效率。消化過程中,可以對(duì)消化懸液進(jìn)行鏡檢,在鏡下觀察到較多的單個(gè)細(xì)胞或70μm以下的細(xì)胞簇后,即可認(rèn)為消化完成。

3.         在確認(rèn)消化完成的組織懸液中加入胎牛血清(Fetal Bovine Serum, FBS)至終濃度達(dá)2-5%后吹打混合均勻以終止消化。

4.         上步驟所獲得消化懸液可直接用于離心或篩網(wǎng)過濾等細(xì)胞分離操作,在使用分離的細(xì)胞前需利用類器官基礎(chǔ)培養(yǎng)基對(duì)樣本進(jìn)行兩次以上的離心清洗方可使用(推薦離心速度200-300g離心3min)。