標簽歸檔：Harlan Teklad

Harlan TD.07108 Zn Defic. Diet (0.5) 鋅缺乏飼料使用說明書

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Harlan TD.07108 Zn Defic. Diet (0.5) 鋅缺乏飼料使用說明書

Harlan Teklad動物飼料
Harlan代理–上海金畔生物科技有限公司
歡迎新老客戶訪問Harlan官網(wǎng)或者咨詢代理上海金畔生物獲取更多詳細資料。

TD.85419? is 0.5 – 1.5 ppm，Diet TD.07108 is a modification of TD.85419 with 0.5 ppm added zinc as zinc sulfate and purple food coloring. Total zinc is estimated to be 2 – 3.5 ppm (background + added zinc)

TD.85419是0.5 – 1.5 ppm，飲食TD.07108是TD.85419的改良形式，添加了0.5 ppm的鋅作為硫酸鋅和紫色食用色素。 總鋅估計為2-3.5 ppm（基礎(chǔ)鋅含量+添加的鋅）

Harlan Teklad Custom Diet
TD.07108 Zn Defic. Diet (0.5)

Formula g/Kg
Egg White Solids, spray-dried 200
Dextrose, monohydrate 634.2376
Corn Oil 100
Cellulose 29.986
Mineral Mix, Zn Deficient (81264) 25.6902
Chromium Potassium Sulfate, dodecahydrate 0.02
Zinc Sulfate, heptahydrate 0.0022
Vitamin Mix, Teklad (40060) 10
Biotin 0.004
Ethoxyquin, antioxidant 0.02
Purple Food Color 0.04

Footnote

A zinc deficient diet with 0.5 ppm added zinc as zinc sulfate and purple food coloring. Total zinc estimated to be 2 – 3.5 ppm (background + added zinc). Modified from TD.85419. For the series TD.07108-TD.07110.

Selected Nutrient Information1

% by weight % kcal from
Protein 16.1 16.6
Carbohydrate 58.3 60.1
Fat 10.0 23.2
Kcal/g 3.9
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Adjusted Zinc
  • Color Coded
  • Zinc Sulfate

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Harlan Teklad 誘導(dǎo)動物NAFLD and NASH飼料

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Harlan Teklad 誘導(dǎo)動物NAFLD and NASH飼料

Teklad diet, bedding and enrichment? ? Teklad laboratory animal diets? ?Custom research diets? ?NAFLD and NASH

誘導(dǎo)嚙齒動物NAFLD/NASH的飲食方法可分為兩大類：

  • 長期喂飼可誘發(fā)肥胖、代謝綜合癥及輕微納什或
  • 短時間喂飼可誘發(fā)嚴重納什的肝臟特征而不引起肥胖或胰島素抵抗的飲食。

下表突出了上述兩個類別的飲食選擇。有關(guān)NAFLD/NASH模型的更完整描述，請參見表后面的下拉菜單。

Dietary methods to induce NAFLD/NASH in rodents can be split into two common categories:

  • diets fed for longer periods of time to induce obesity, metabolic syndrome, and mild NASH or
  • diets fed for short periods of time to induce hepatic features of severe NASH without inducing obesity or insulin resistance

This page provides further information on dietary methods to induce NAFLD/NASH.?We’ve also prepared a?downloadable?NASH/NAFLD mini paper.

The tables below highlight diet options from both of the above categories. For more complete descriptions of NAFLD/NASH models see the drop down menus that follow the tables.

Diet options for inducing obesity, metabolic syndrome and mild NAFLD/NASH
Diet features Western/Fast Food ALIOS FPC diet
Product Code TD.88137 TD.06303 TD.160785?PWD?dough

TD.190142?pellet
Fat, % Kcal 42 45 52
Fat Sources,
% by weight		 21% milk fat 22%?hydrogenated vegetable oil
1% soybean oil		 19%?hydrogenated vegetable oil
6% milk fat
4% palmitic acid
Fatty acid profile,
% total fat		 66% saturated
30% monounsaturated
4% polyunsaturated		 23% saturated
31% monounsaturated (cis)
12% polyunsaturated (cis)
34% trans		 43% saturated
27% monounsaturated (cis)
7% polyunsaturated (cis)
23% trans
Sugars, % by weight 34.5% sucrose 22.4% sucrose 34.5% sucrose
Cholesterol, % by weight 0.2 0 1.25
Modifications TD.96121?1.25% cholesterol
TD.120528?Increased sucrose, 1.25% cholesterol		 TD.120330?0.2% cholesterol
TD.130885?0.2% cholesterol, 27% sucrose		 TD.140154?adds customer supplied palmitic acid

For high fat diet options to induce uncomplicated NAFLD see our?Diet Induced Obesity page.

Diet options for inducing more severe hepatic NAFLD/NASH without obesity or metabolic syndrome
Diet features High Fat, Cholesterol & Cholate Methionine/choline deficient (MCD)
Product Code TD.02028 TD.90262
Fat, % Kcal 42 22
Fat Sources,
% by weight		 21% milk fat 10% corn oil
Fatty acid profile,
% total fat		 66% saturated
30% monounsaturated
4% polyunsaturated		 14% saturated
28% monounsaturated
58% polyunsaturated
Sugars, % by weight 33.3% sucrose 46% sucrose
Cholesterol, % by weight 1.25 0
Cholate Source, % by weight 0.5 0
Related diets TD.09237?15% milk fat, 1% cholesterol
TD.88051?Hybrid version		 TD.94149?MCD control diet

Diets inducing obesity, metabolic syndrome and mild NAFLD/NASH

Western or fast food style diets fed to induce NASH with metabolic syndrome contain 40 – 45% kcal from milkfat (a fat source high in palmitate) with added cholesterol (0.15 – 2%) and are high in sucrose (>30%). Dietary palmitate and cholesterol have both previously been associated with the progression from simple steatosis to NASH.

Examples:

  • TD.88137?? ? ? Adjusted Calories Diet (42% from fat)
  • TD.96121?? ? ? 21% MF, 1.25% Chol. Diet
  • TD.120528? ? ?42% Kcal/Fat Diet (Incr. Sucrose, 1.25% Chol.)

Research use:

These diets can induce obesity, metabolic syndrome, and simple steatosis within nine weeks of feeding. Increased hepatic inflammation has been observed after 12 weeks of feeding. NASH typically requires longer feeding with fibrosis developing within nine months and late stage fibrosis including hepatic ballooning occurring after 14 – 20 months of feeding. Increasing dietary sucrose (~41%) and cholesterol (~1.25%) accelerates the NASH phenotype with steatosis, inflammation and hepatocyte ballooning observed within 12 weeks. In addition to feeding a high fat diet, providing a glucose/fructose mixture in the drinking water may further promote NASH development.

Select References:

Charlton, M., et al., Fast food diet mouse: novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest Liver Physiol, 2011. 301(5): p. G825-34.?http://www.ncbi.nlm.nih.gov/pubmed/21836057

Gores, G., Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, Masuoko H. Am J Physiol Gastrointest Liver Physiol, 2015. 308: p. G159.?http://ajpgi.physiology.org/content/308/2/G159

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44.?http://www.ncbi.nlm.nih.gov/pubmed/19119140

Ioannou, G.N., et al., Hepatic cholesterol crystals and crown-like structures distinguish NASH from simple steatosis. J Lipid Res, 2009. 54(5): p. 1326-34.?http://www.ncbi.nlm.nih.gov/pubmed/23417738

Alkhouri, N., et al., Adipocyte apoptosis, a link between obesity, insulin resistance, and hepatic steatosis. J Biol Chem, 2010. 285(5): p. 3428-38.?http://www.ncbi.nlm.nih.gov/pubmed/19940134

Dixon, L.J., et al., Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One, 2013. 8(2): p. e56100.?http://www.ncbi.nlm.nih.gov/pubmed/23409132

DeLeve, L.D., et al., Prevention of hepatic fibrosis in a murine model of metabolic syndrome with nonalcoholic steatohepatitis. Am J Pathol, 2008. 173(4): p. 993-1001.?http://www.ncbi.nlm.nih.gov/pubmed/18772330

VanSaun, M.N., et al., High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol, 2009. 175(1): p. 355-64.?http://www.ncbi.nlm.nih.gov/pubmed/19541928

Asgharpour, A., et al., A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. J Hepatol, 2016. 65(3): p. 579-88.?http://www.ncbi.nlm.nih.gov/pubmed/27261415

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95.?http://www.ncbi.nlm.nih.gov/pubmed/18772365

Tsuchida, T., et al., A simple diet-and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer. Journal of hepatology, 2018. 69(2):385-395.?https://www.ncbi.nlm.nih.gov/pubmed/29572095

The American Lifestyle-Induced Obesity Syndrome (ALIOS) model involves feeding the “American fast food” diet high in trans-fats and sugar. Dietary trans-fats from hydrogenated vegetable shortening (HVO) are associated with increased insulin resistance and hepatic inflammation in rodent NASH models. In addition to diet, a glucose/fructose solution is added to the drinking water and sedentary behavior promoted by removing the overhead cage feeders in this model.

Examples:

  • TD.06303?? ? ??22% HVO Diet
  • TD.120330?? ??22% HVO + 0.2% Cholesterol Diet
  • TD.130885?? ??ALIOS with Added Sugar

Research use:

The ALIOS model develops obesity with insulin resistance, elevated ALT levels, and steatosis within 16 weeks. Increased inflammation and early development of fibrosis have been observed at 6 months. Severe steatosis with fibrosis and inflammation develops within 12 months of feeding with 50% of the mice reportedly developing hepatic neoplasms. Adding cholesterol (0.2%) to the American Fast Food diet may accelerate NASH phenotype development.

Select References:

Koppe, S.W., et al., Trans fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared with a standard murine high-fat diet. Am J Physiol Gastrointest Liver Physiol, 2009. 297(2): p. G378-84.?http://www.ncbi.nlm.nih.gov/pubmed/19541924

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95.?http://www.ncbi.nlm.nih.gov/pubmed/18772365

Mells, J.E., et al., Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol, 2012. 302(2): p. G225-35.?http://www.ncbi.nlm.nih.gov/pubmed/22038829

Dowman, J.K, et al., Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle. Am J Pathol, 2014. 184(5):1550-1561.?https://www.ncbi.nlm.nih.gov/pubmed/24650559?

Mells, J.E., et al., Saturated fat and cholesterol are critical to inducing murine metabolic syndrome with robust nonalcoholic steatohepatitis. J Nutr Biochem, 2014. 26(3): p. 285-92.?http://www.ncbi.nlm.nih.gov/pubmed/25577467

The Fructose, Palmitate, Cholesterol and Trans-Fat (FPC) diet is a recent NASH diet that includes Western and ALIOS model diets to achieve both metabolic and hepatic NASH features within an accelerated time frame. Key features of the FPC diet include 1) a lower Met content than typical rodent diets by decreasing total protein without supplementing sulfur amino acids; 2) choline supplementation is lower than typical but is not considered deficient; 3) high in sucrose (~34% by weight); 4) 1.25% cholesterol; 5) 52% kcal from fat with fat sources including milkfat fat, palmitic acid and hydrogenated vegetable shortening to provide trans-fats. Like the ALIOS model, the FPC model also provides a glucose/fructose solution to the drinking water.

Examples:

  • TD.160785?? ??52 kcal/Fat Diet (C16:0, HVO, AMF, Choline/Met)

Research use:

Male C57BL/6J mice fed the FPC diet and provided a glucose/fructose drinking solution developed insulin resistance and NAFLD with inflammation, hepatocyte death, and fibrosis within 16 weeks.

Select References:

Wang, X., et al., Hepatocyte TAZ/WWTR1 promotes inflammation and fibrosis in nonalcoholic steatohepatitis. Cell Metab, 2016. 24(6): p. 848-62.?https://www.ncbi.nlm.nih.gov/pubmed/28068223??

Zhu, C., et al., Hepatocyte Notch activation induces liver fibrosis in nonalcoholic steatohepatitis. Sci Transl Med, 2018. 10(468).?https://www.ncbi.nlm.nih.gov/pubmed/30463916

Common diets to induce obesity (DIO) can be fed to induce uncomplicated NAFLD. These high fat diets typically contain 40–60% kcal from fat without supplemented cholesterol or cholate. Simple sugars such as sucrose or fructose can also be supplemented via diet or water to progress the fatty liver phenotype. Diets can be in pellet or powder/dough form depending on the formula. Some models require limited physical activity and in those cases diets can be fed inside the cage. For more information see our?Diet Induced Obesity?page.

Examples:

  • TD.08811?? ? ??45%kcal Fat Diet (21% MF, 2% SBO)
  • TD.06414?? ? ??Adjusted Calories Diet (60/Fat)

Research use:

In susceptible rodent models, high fat diets are commonly used to induce NAFLD with obesity and insulin resistance common metabolic features associated with NASH in humans. However, the degree of NASH pathology (steatosis, inflammation, and fibrosis) is limited or mild and varies depending on the animal model, length of feeding, and dietary components.

Diets to induce severe hepatic NAFLD/NASH without obesity or metabolic

COLLAPSE ALL TABS

Originally formulated to induce mild atherosclerosis in wild-type rodents, high fat diets containing added cholesterol (1 – 1.25%) and cholate (0.5% as sodium cholate or cholic acid) have also been useful in inducing NASH. This diet option includes purified “Western” style diets with increased cholesterol and cholate and also hybrid diets. Hybrid diets were originally developed by Beverly Paigen and colleagues by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate) resulting in a diet containing ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. Although a less refined approach, the hybrid diet is associated with increased gallstone formation and liver damage as compared to similar purified diets.

Examples:

  • TD.02028?? ? ??Atherogenic Rodent Diet
  • TD.88051?? ? ??Cocoa Butter Diet and Purina Mouse Chow
  • TD.09237?? ? ??15% AMF Diet (1% Chol, 0.5% NaChol)

Research use:

Atherogenic diets are able to induce varied degrees of NASH with increased hepatic inflammation with early fibrosis observed after ten weeks of feeding. However, the metabolic profile typical in human NASH (obesity with insulin resistance) is not recapitulated in this model with animals typically maintaining similar body weights as control fed groups without the development of metabolic syndrome.

Select References:

Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.?http://www.ncbi.nlm.nih.gov/pubmed/2380634

Kamari, Y., et al., Lack of interleukin-1alpha or interleukin-1beta inhibits transformation of steatosis to steatohepatitis and liver fibrosis in hypercholesterolemic mice. J Hepatol, 2011. 55(5): p. 1086-94.?http://www.ncbi.nlm.nih.gov/pubmed/21354232

Kim, D.G., et al., Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model. J Neuroinflammation, 2016. 13: p. 1.?http://www.ncbi.nlm.nih.gov/pubmed/26728181

Madrigal-Perez, V.M., et al., Preclinical analysis of nonsteroidal anti-inflammatory drug usefulness for the simultaneous prevention of steatohepatitis, atherosclerosis and hyperlipidemia. Int J Clin Exp Med, 2015. 8(12): p. 22477-83.?http://www.ncbi.nlm.nih.gov/pubmed/26885230

Savransky, V., et al., Chronic intermittent hypoxia causes hepatitis in a mouse model of diet-induced fatty liver. Am J Physiol Gastrointest Liver Physiol, 2007. 293(4): p. G871-7.?http://www.ncbi.nlm.nih.gov/pubmed/17690174

Methionine and choline deficient (MCD) diets are amino acid defined rodent diets deficient in methionine and choline, high in sucrose (>40% by weight) with ~10% corn oil by weight. Methionine and choline deficiency decreases fat oxidation and export of fat from the liver. Dietary sucrose is necessary for hepatic lipid accumulation and oxidation. The polyunsaturated fat in corn oil promotes hepatic lipid oxidation.

Example:

  • TD.90262?? ? ??Methionine/Choline Deficient Diet

Control:

  • TD.94149?? ? ??Amino Acid Control Diet

Research use:

Steatosis, increased serum alanine aminotransferase (ALT), inflammation, and hepatic fat oxidation has been observed within three weeks of feeding the MCD diet with fibrosis development after six weeks. This dietary model does not produce metabolic syndrome (an aspect of NASH in human models) and progressive weight loss (up to 40%) is associated with the MCD diet feeding.

蛋氨酸/膽堿缺乏癥(MCD)日糧
蛋氨酸和膽堿缺乏(MCD)飼料是一種氨基酸定義的嚙齒動物飼料，缺乏蛋氨酸和膽堿，蔗糖含量高(體重>40%)，玉米油含量約10%。蛋氨酸和膽堿缺乏減少脂肪氧化和從肝臟輸出脂肪。日糧蔗糖對肝臟脂質(zhì)的積累和氧化是必需的。玉米油中的多不飽和脂肪促進肝臟脂質(zhì)氧化。

例子：TD.90262 蛋氨酸/膽堿缺乏癥飲食
管制：TD.94149 氨基酸控制飲食

研究用途：

觀察到脂肪變性、血清丙氨酸轉(zhuǎn)氨酶(ALT)升高、炎癥和肝脂肪氧化反應(yīng)。這種飲食模式不會產(chǎn)生代謝綜合癥(在人類模型中是NASH的一個方面)和累進性減肥(高達40%)與MCD飲食喂養(yǎng)有關(guān)。

Select References:

Pickens, M.K., et al., Dietary sucrose is essential to the development of liver injury in the MCD model of steatohepatitis. J Lipid Res, 2009. 50(10):2072-82.??http://www.ncbi.nlm.nih.gov/pubmed/19295183

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44.?http://www.ncbi.nlm.nih.gov/pubmed/19119140

Lee, G.S., et al., Polyunsaturated fat in the methionine-choline-deficient diet influences hepatic inflammation but not hepatocellular injury. J Lipid Res, 2007. 48(8): p. 1885-96.?http://www.ncbi.nlm.nih.gov/pubmed/17526933

Vetelainen, R., A. van Vliet, and T.M. van Gulik, Essential pathogenic and metabolic differences in steatosis induced by choline or methione-choline deficient diets in a rat model. J Gastroenterol Hepatol, 2007. 22(9): p. 1526-33.?http://www.ncbi.nlm.nih.gov/pubmed/17716355

Leclercq, I.A., et al., Intrahepatic insulin resistance in a murine model of steatohepatitis: effect of PPARgamma agonist pioglitazone. Lab Invest, 2007. 87(1): p. 56-65.?http://www.ncbi.nlm.nih.gov/pubmed/17075577

Kashireddy, P.R. and M.S. Rao, Sex differences in choline-deficient diet-induced steatohepatitis in mice. Exp Biol Med (Maywood), 2004. 229(2): p. 158-62.?http://www.ncbi.nlm.nih.gov/pubmed/14734794

Dixon, L.J., et al., Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis. Lab Invest, 2012. 92(5): p. 713-23.?http://www.ncbi.nlm.nih.gov/pubmed/22411067

Dietary models of NAFLD/NASH continue to evolve with the goal of more accurately recapitulating both the metabolic and hepatic symptoms of human disease. Commonly researchers are studying the synergistic effects of various NASH dietary features to accelerate progression of the model and severity of liver disease.

A Teklad nutritionist can work with you to formulate new diets in order to investigate novel dietary models of NAFLD/NASH.

The choice of control diet is dependent on the specific research goal. Many researchers choose to compare their NAFLD/NASH diet-fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diet or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens).

Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component. In some cases, such as those studies feeding amino acid defined diets like the MCD model, a matched control diet is recommended given the very different formulations and protein sources of grain-based diets.

When making inferences about specific nutrients within the diet an ingredient matched, low fat control diet may be necessary. There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined and digestible) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible).

A very basic purified control diet would be AIN-93M?TD.94048?or AIN-93G?TD.94045. AIN-93 diets have a moderate amount of sucrose at ~10% with fat from soybean oil providing a healthy fatty acid profile.

Contact a nutritionist?for an additional information and control diet recommendations.

對照日糧
控制飲食的選擇取決于具體的研究目標。許多研究人員選擇比較他們的NAFLD/納什飲食喂養(yǎng)的動物和喂養(yǎng)一種天然成分的動物，谷物為基礎(chǔ)的飲食(也稱為標準飲食或周食)。這些飲食在營養(yǎng)來源和水平以及非營養(yǎng)因素(如植酸鹽或植物雌激素)存在的情況下存在差異。

根據(jù)您的主要比較，它可能適合作為您的對照/參考組谷物基礎(chǔ)的飲食。然而，這樣的比較限制了對飲食模式和特定飲食成分的推斷。在某些情況下，如那些研究喂食氨基酸定義的飲食，如mcd模型，建議一個匹配的對照飲食，考慮到非常不同的配方和蛋白質(zhì)來源的谷物為基礎(chǔ)的飲食。

當(dāng)對飲食中的特定營養(yǎng)成分做出相應(yīng)的推斷時，低脂控制飲食可能是必要的。除了不同類型的碳水化合物外，還有許多不同水平和類型的脂肪，從蔗糖(高精制和可消化)到玉米淀粉(精制，但更復(fù)雜)，再到抗性淀粉(精制，但不能完全消化)。

一種非?；镜募冋刂骑嬍硨⑹?9300萬。TD.94048或者是93g TD.94045。AIN-93日糧中含有適量的蔗糖~10%，大豆油中的脂肪提供了健康的脂肪酸譜。

Need more information? A Teklad nutritionist will work with you to determine if existing diets will meet your needs or formulate new diets to help you investigate novel dietary models of NAFLD/NASH.?Contact us?for a diet consultation.

Harlan Teklad TD.08485 Low Fat Control Diet低脂飼料

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Harlan Teklad TD.08485 Low Fat Control Diet低脂飼料

Harlan Teklad代理，Harlan?飼料代理，歡迎訪問Harlan Teklad官網(wǎng)或者咨詢我們獲取更多相關(guān)產(chǎn)品信息。

Control diets can be designed in several ways, depending on what features the researcher wants to modify relative to the high-fat diet. These are just a few examples.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat

Formula g/Kg
Casein 195
DL-Methionine 3
Sucrose 120
Corn Starch 432.99
Maltodextrin 100
Anhydrous Milkfat 37.2
Soybean Oil 12.8
Cellulose 50
Mineral Mix, AIN-76 (170915) 35
Calcium Carbonate 4
Vitamin Mix, Teklad (40060) 10
Ethoxyquin, antioxidant 0.01

Footnote

This is modified from TD.88137 to reduce fat, reduce sucrose, and remove cholesterol.

elected Nutrient Information1

 

% by weight % kcal from
Protein 17.3 19.1
Carbohydrate 61.3 67.9
Fat 5.2 13.0
Kcal/g 3.6
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Modification of TD.88137
  • Low Fat & No Added Cholesterol
  • Reduced Sucrose

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

 

Harlan TD.00158 維生素A缺乏飲食 Vitamin A Deficient Diet 使用說明

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Harlan TD.00158 維生素A缺乏飲食 Vitamin A Deficient Diet

貨號：TD.00158

英文名稱：Vitamin A Deficient Diet

Formula g/Kg
Casein,”iamin-Freet”Test 200
L-Cystine 3
Com?Starch 397.486
Maltodextrin 132
Sucrose 109.755
Soybean?Oil 70
Cellulose 50
Mineral?Mix,?AIN-93G-MX?(94046) 35
Choline?Bitartrate 2.5
TBHQ,?antioxidant 0.014
Niacin 0.03
Calcium?Pantothenate 0.016
Pyridoxine?HCI 0.007
Thiamin?(81%) 0.006
Riboflavin 0.006
Folic?Acid 0.002
Biotin 0.0002
Vitamin?B.2?(0.1%?in?mannitol) 0.025
Vitamin?E?DL-alpha?tocooheryl?acetate?(500?IU/g) 0.15
Vitamin?D3?cholecalciferol?(500,000?lU/g) 0.002
Vitamin?K1.?phyloquinone 0.0008

Footnote
This modification of diet AIN-93G (TD 94045) omits vitamin A palmitate and
substitutes alcohol-extracted casein for regular casein. Diet should contain
less than 20 IU vitamin AVkg diet.

Selected Nutrient Information

%?by?weight %?kcal?from
Protein 18.3 19.4
Carbohydrate 60.1 63.8
Fat 7 16.7
Kcalg 3.8

Key Features
+ Purified Diet
+ Vitamin A
+ VFT Casein
+ AIN-93G Modification

Key Planning Information
+ Products are made fresh to order
+ Store product at 4°C or lower? ?將產(chǎn)品存放在4°C或更低的溫度下

+Use within 6 months (applicable to most diets)
+ Box labeled with product name,
manufacturing date, and lot number
+ Replace diet at minimum once per week
More frequent replacement may be advised

+ Lead time:
2 weeks non-irradiated
4 weeks irradiated

Product Specific Information
1/2”Peilet of Powder (free flowing)? 1/2”粉餅（自由流動）
+ Minimum order 3 Kg
+ Irradiation not advisea
Contact a nutntionist for recommendations

Harlan Teklad 動物飼料 Vitamin A Diet(25,000)成分表

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Harlan Teklad 動物飼料 Vitamin A Diet(25,000)成分表

Harlan Teklad動物飼料
Harlan代理–上海金畔生物科技有限公司
歡迎新老客戶訪問Harlan官網(wǎng)或者咨詢中國代理商上海金畔生物獲取更多詳細資料。

Formula g/Kg
Casein,?“Vitamin-Free”Test 200
L-Cystine 3
Com?Starch 397.486
Maltodextrin 132
Sucrose 109.705
Soybean Oil 70
Cellulose 50
Mineral?Mix,?AIN-93G-MX?(94046) 35
Choline?Bitartrate 2.5
TBHQ,?antioxidant 0.014
Niacin 0.03
Calcium?Pantothenate 0.016
Pyridoxine?HCI 0.007
Thiamin?(81%) 0.006
Riboflavin 0.006
Folic?Acid 0.002
Biotin 0.0002
Vitamin?B12?(0.1%?in?manitol) 0.025
Vitamin?E?DL?alpha?tocopheryl?acetate?(500?IU/g) 0.15
Vitamin?D3.?cholecalciferol?(500,000?IU/g) 0.002
Vitamin?K1,?phylloquinone 0.0008
Vitamin?A?Acetate?(500,000?IU/g) 0.05

Foothote

This modification of diet AIN-93G (TD.94045) replaces vitamin A palmitate with
tamin A acetate and increases the total concentration to approximately 25,000
/kg. Alcohol-extracted casein is substituted for regular casein.

Selected Nutrient Information

%?by?weight %?kcal?from
Protein 18.3 19.4
Carbohydrate 60.1 63.8
Fat 7 16.7
Kcal/g 3.8

Values are calculated from ingredient analysis or manufacturer data

Key Features

+ Purifield? ?Diet
+Vitamin A
+AlN 93G Modification
+ Rodent

Key Planning Information

+ Product are made Fresh fo order
+ store product at 4° C or IoWer
+ Use within 6 months(appIicable to most diets)
+ Box Labeled with product name
manufaGturing date,and Iot number
+ Replace diet at nninimunη once per weekation

Harlan動物飼料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 成分表

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Harlan動物飼料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 成分表

Harlan Teklad動物飼料
Harlan代理–上海金畔生物科技有限公司
歡迎新老客戶訪問Harlan官網(wǎng)或者咨詢中國代理商上海金畔生物獲取更多詳細資料。

嚙齒動物飼料（2018，625強力霉素）

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 999.375
Doxycycline Hyclate 0.625

Footnote? ?Harlan動物飼料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 使用說明

This diet was designed to deliver a daily dose of 2-3 mg of doxycycline based on consumption of 4-5 g/d by a mouse. Doxycycline hyclate contains approximately 87% doxycycline.

這種飲食被設(shè)計為以小鼠每天4-5 g / d的消耗量提供2-3 mg強力霉素的日劑量。 鹽酸多西環(huán)素含有約87％的多西環(huán)素。

Selected Nutrient Information

  % by weight % kcal from
Protein 18.2 22.9
Carbohydrate2 48.0 60.5
Fat 5.8 16.6
Kcal/g 3.2  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Key Features

  • Standard Diet Base
  • Doxycycline
  • Gene Induction
  • 2018

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Harlan Teklad Diet ingredients

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Harlan Teklad Diet ingredients

Teklad diet, bedding and enrichment?+?Teklad laboratory animal diets?+?Custom research diets?+?Diet ingredients

You may want to prepare your own diet at your research site or facility, and if that is the case, you depend on the ingredients being of the highest quality. Envigo can provide you with many of the same ingredients used to produce our advanced Teklad custom research diets so you can develop your own formula. And as always, our nutritional experts are here to help you make the best decisions for your ingredients and ultimate formulation.

Our ingredients

We offer several?ingredients?for individual sale so that you can develop your own blends.

“VFT” casein

  • “Vitamin-free” test casein?(alcohol-extracted) is best suited for purified test diet formulas where fat or vitamin content needs to be precisely controlled
  • The reduced levels of many vitamins in VFT casein make it the preferred protein source for many vitamin studies. Thus, the nickname “Vitamin-free” was given to the extracted casein many years ago

Vitamin mixes

  • The common vitamin mixes shown below use sucrose or corn starch as a carrier
  • Customized vitamin mixes may use cellulose if a non-nutritive carrier is necessary
  • Vitamin mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of your laboratory animal
  • For those preparing their own diets, vitamin premixes are an efficient way to add vitamins to a diet
  • Vitamin mixes should be stored at 4 degrees celsius or lower upon receipt. Storage at –20 degrees celsius is recommended if the mix will be used beyond 1 month
  • Minimum order is 500 g for both stock and custom vitamin mixes
  • Our available vitamin mixes are commonly used in diets, and are available from stock
  • Vitamin mixes are shipped within a few days of order

Our Formula examples

  • CA.40060?Teklad vitamin mix
  • CA.40077?AIN-76A vitamin mix
  • TD.94047?AIN-93 vitamin mix

Mineral mixes

  • The common mineral mixes shown below as examples use sucrose as a carrier
  • Customized mineral mixes could use cellulose or no carrier if a non-nutritive carrier is necessary
  • Our mineral mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of the animal
  • For those preparing their own diets, mineral premixes are an efficient way to add minerals to a diet
  • Mineral mixes should be stored in a dry cool environment and used within a year of receipt. The minimum order is 500 g for both stock and custom mineral mixes
  • These mineral mixes are commonly used in diets, and are available from stock
  • Our mineral mixes are shipped within a few days of order

Formula examples:

  • CA.170915?AIN-76 mineral mix
  • TD.94046?AIN-93G mineral mix
  • TD.94049?AIN-93M mineral mix

Other mixes (may or may not be available from stock):

  • TD.83171?Vitamin mix without A, D, E, choline
  • TD.81062?Iron deficient mineral mix based on AIN-76A
  • TD.79055?Calcium and phosphorus deficient mineral mixed based on AIN-76
  • TD.98057?Calcium and phosphorus deficient mineral mixed based on AIN-93
  • CA.170760?Rogers-Harper mineral mix

你可能想要在你的研究地點或設(shè)施準備你自己的飲食，如果是這樣的話，你取決于成分是最高的質(zhì)量。Envigo可以為您提供許多相同的成分，用于生產(chǎn)我們先進的特克拉德定制研究飲食，以便您可以開發(fā)自己的配方。和往常一樣，我們的營養(yǎng)專家在這里幫助您為您的配料和最終配方做出佳決定。

我們的配料

我們提供幾個配料供個人銷售，以便您可以開發(fā)自己的混合。

“VFT”酪蛋白

  • “無維生素”測試酪蛋白(酒精提取)最適合于那些需要精確控制脂肪或維生素含量的純正試驗飲食配方。
  • VFT酪蛋白中許多維生素含量的降低使得它成為許多維生素研究的首選蛋白質(zhì)來源。因此，“無維生素”的綽號是在許多年前給提取出來的酪蛋白起的。

維生素混合物

  • 以下常見的維生素混合物使用蔗糖或玉米淀粉作為載體。
  • 定制的維生素混合物可能使用纖維素，如果非營養(yǎng)載體是必要的。
  • 維生素混合物的制定考慮到了飲食中的包容率。多用或少用會損害你的實驗動物的健康。
  • 對于那些自己準備飲食的人來說，維生素預(yù)混劑是向飲食中添加維生素的一種有效方法。
  • 維生素混合物應(yīng)儲存在4攝氏度或更低的接收。如果使用時間超過1個月，建議使用攝氏-20度。
  • 庫存和定制維生素混合物的最低訂購量為500克。
  • 我們可用的維生素混合物通常用于飲食，并可從庫存中獲得。
  • 維生素混合物在訂購后幾天內(nèi)發(fā)貨。

我們的公式示例

  • CA.40060 特克拉德維生素混合物
  • CA.40077 AIN-76A維生素混合物
  • TD.94047 AIN-93維生素混合物

礦物混合物

  • 下面所示的常見礦物混合物是以蔗糖為載體的。
  • 如果需要非營養(yǎng)的載體，定制的礦物混合物可以使用纖維素，也可以不使用載體。
  • 我們的礦物質(zhì)混合物是根據(jù)飲食包容率來制定的。多用或少用會損害動物的健康。
  • 對于那些自己準備飲食的人來說，礦物預(yù)混料是向飲食中添加礦物質(zhì)的有效方法。
  • 礦物混合物應(yīng)存放在干燥、涼爽的環(huán)境中，并在收到后一年內(nèi)使用。庫存和自定義礦物混合物的最低訂購量為500克。
  • 這些礦物混合物通常用于飲食，并可從庫存中獲得。
  • 我們的礦物混合物在訂貨后幾天內(nèi)裝運。

公式示例：

  • CA.170915 AIN-76礦物混合物
  • TD.94046 AIN-93g礦物混合物
  • TD.94049 AIN-9300萬礦物混合物

其他混合材料(可能從庫存中獲得，也可能無法從庫存中獲得)：

  • TD.83171不含A，D，E，膽堿的維生素混合物
  • TD.81062基于AIN-76A的缺鐵礦物配料
  • TD.79055?AIN-76混合鈣磷缺乏礦物
  • TD.98057AIN-93混合鈣磷缺乏礦物
  • CA.170760 羅杰斯-哈珀礦物混合物

Harlan Teklad 2016 global 16% protein rodent diets

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Harlan Teklad 2016 global 16% protein rodent diets

Product Features

  • Designed to support growth and maintenance
  • Formulated to exclude soybean meal, thus minimizing the presence of isoflavones, the primary type of?phytoestrogen?found in lab animal diets
  • Typical isoflavone concentrations (daidzein + genistein aglycone equivalents) range from non-detectable to 20 mg/kg
  • Exclusion of alfalfa reduces chlorophyll, greatly improving?fluorescent optical imagingclarity
  • Absence of animal protein and fish meal minimizes the presence of nitrosamines (a potential carcinogen)
Products
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 16% protein 2016, 2016C 2016S 2916
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.

Teklad Global 16% Protein Rodent Diet

Product Description- 2016 is a fixed formula, non-autoclavable diet
manufactured with high quality ingredients and designed to support growth
and maintenance. 2016 does not contain alfalfa or soybean meal, thus
minimizing the occurrence of natural phytoestrogens. Typical isoflavone
concentrations (daidzein + genistein aglycone equivalents) range from nondetectable
to 20 mg/kg. Exclusion of alfalfa reduces chlorophyll, improving
optical imaging clarity. Absence of animal protein and fish meal minimizes
the presence of nitrosamines. Also available certified (2016C) and
irradiated (2916). For autoclavable diet, refer to 2016S (Sterilizable).

Macronutrients

Crude Protein % 16.4
Fat (ether extract) a % 4.0
Carbohydrate (available) b % 48.5
Crude Fiber % 3.3
Neutral Detergent Fiber c % 15.2
Ash % 4.9
Energy Density d kcal/g (kJ/g) 3.0 (12.6)
Calories from Protein % 22
Calories from Fat % 12
Calories from Carbohydrate % 66
Minerals    
Calcium % 1.0
Phosphorus % 0.7
Non-Phytate Phosphorus % 0.4
Sodium % 0.2
Potassium % 0.6
Chloride % 0.4
Magnesium % 0.2
Zinc mg/kg 70
Manganese mg/kg 100
Copper mg/kg 15
Iodine mg/kg 6
Iron mg/kg 200
Selenium mg/kg 0.23
Amino Acids    
Aspartic Acid % 1.0
Glutamic Acid % 3.3
Alanine % 0.9
Glycine % 0.7
Threonine % 0.6
Proline % 1.5
Serine % 0.8
Leucine % 1.9
Isoleucine % 0.7
Valine % 0.8
Phenylalanine % 0.9
Tyrosine % 0.5
Methionine % 0.3
Cystine % 0.3
Lysine % 0.8
Histidine % 0.4
Arginine % 0.8
Tryptophan % 0.2

Teklad Diets are designed and manufactured for research purposes only.

Ingredients (in descending order of inclusion)- Ground wheat, ground corn,?wheat middlings, corn gluten meal, calcium carbonate, dicalcium phosphate, soybean oil, brewers dried yeast, iodized salt, L-lysine, DL-methionine, choline chloride, magnesium oxide, vitamin E acetate, menadione sodium bisulfite complex (source of vitamin K activity), manganous oxide, ferrous sulfate, zinc oxide, niacin, calcium pantothenate, copper sulfate, pyridoxine hydrochloride, riboflavin, thiamin mononitrate, vitamin A acetate, calcium iodate, vitamin B12?supplement, folic acid, biotin, vitamin D3?supplement,

cobalt carbonate.

Vitamins

Vitamin A e, f IU/g 15.0
Vitamin D3?e, g IU/g 1.5
Vitamin E IU/kg 110
Vitamin K3?(menadione) mg/kg 50
Vitamin B1?(thiamin) mg/kg 17
Vitamin B2?(riboflavin) mg/kg 15
Niacin (nicotinic acid) mg/kg 75
Vitamin B6?(pyridoxine) mg/kg 18
Pantothenic Acid mg/kg 33
Vitamin B12?(cyanocobalamin) mg/kg 0.08
Biotin mg/kg 0.40
Folate mg/kg 4
Choline mg/kg 1030
Fatty Acids    
C16:0 Palmitic % 0.5
C18:0 Stearic % 0.1
C18:1ω9 Oleic % 0.7
C18:2ω6 Linoleic % 2.0
C18:3ω3 Linolenic % 0.1
Total Saturated % 0.6
Total Monounsaturated % 0.7
Total Polyunsaturated % 2.1
Other    
Cholesterol mg/kg —

 

  • Ether extract is used to measure fat in pelleted diets, while an acid hydrolysis method is required to recover fat in extruded diets. Compared to ether extract, the fat value for acid hydrolysis will be approximately 1% point higher.
  • Carbohydrate (available) is calculated by subtracting neutral detergent fiber from total carbohydrates.
  • Neutral detergent fiber is an estimate of insoluble fiber, including cellulose, hemicellulose, and lignin. Crude fiber methodology underestimates total fiber.
  • Energy density is a calculated estimate of metabolizable energybased on the Atwater factors assigning 4 kcal/g to protein, 9 kcal/g to fat, and 4 kcal/g to available carbohydrate.
  • Indicates added amount but does not account for contribution from other ingredients.
  • 1 IU vitamin A = 0.3 μg retinol
  • 1 IU vitamin D = 25 ng cholecalciferol

For nutrients not listed, insufficient data is available to quantify.

Nutrient data represent the best information available, calculated from published values and direct analytical testing of raw materials and finished product. Nutrient values may vary due to the natural variations in the ingredients, analysis, and effects of processing.

Harlan 嚙齒類飼料 Rodent diets

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Harlan 嚙齒類飼料 Rodent diets

As a researcher, you want to have as much control as possible over what goes into your study animals. At Envigo, we understand that. That is why we developed?Teklad Global Rodent Diets?.

Teklad Global Rodent Diets??are a special integrated range of vegetarian laboratory rodent diets developed to be nutritionally complete for various life stages from breeding through long-term maintenance. Global rodent laboratory diets contain levels of protein, energy, vitamins and minerals that are more appropriate to the needs of modern biomedical research studies.

Furthermore, in global rodent diets particular attention has been placed on avoiding, as far as practical, ingredients that are reported to have adverse confounding effects on experimental results. This has resulted in a range of Teklad rodent laboratory diets that contain:

No fish meal

No meat meals or meat by-products

No alfalfa meal

No soybean meal or reduced levels

No animal fat

By excluding animal by-products, the presence of nitrosamines (a potential carcinogen) is avoided. Exclusion of alfalfa meal reduces chlorophyll, improving optical imaging clarity. Reduction or removal of soybean meal, together with elimination of alfalfa meal, minimizes levels of naturally-occurring phytoestrogens. Phytoestrogens interact with endogenous estrogens and potentially can affect studies in many?research areas. Read more about this in these pieces:

Dietary phytoestrogens, a source of research variation
Ask a Nutritionist Series: Impact of phytoestrogens on research, Volume 1

Phytoestrogens limit translation of preclinical results to clinical outcomes
Ask a Nutritionist Series: Impact of phytoestrogens on research, Volume 2

Products
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 14% protein 2014, 2014C 2014S 2914
Teklad global 16% protein 2016, 2016C 2016S 2916
Teklad global 18% protein 2018, 2018C 2018S, 2018SX 2918
Teklad global 19% protein extruded 2019 2019S 2919
Teklad global soy protein-free extruded 2020X 2020SX 2920X
Traditional diets see more
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.
產(chǎn)品
Teklad嚙齒類食物 不可蒸壓式 高壓釜 輻照
總蛋白14% 2014 2014C 2014S 2914
總蛋白16% 2016 2016C 2016S 2916
Teklead全局18%蛋白 2018 2018C 2018S，2018SX 2918
Teklad 19%蛋白擠出 2019 2019S 2919
Teklad 無大豆蛋白擠出 2020 X 2020SX 2920 X
傳統(tǒng)飲食 見更多
并不是所有的產(chǎn)品都是在當(dāng)?shù)貛齑娴?，可能需要延長交貨期和額外的費用。

Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer global diet line for your modern research needs.

Teklad Global Rodent Diets??are modern formulas designed to reduce research variables. Specifically, these diets contain more appropriate nutrient levels, and limit or exclude ingredients that are reported to have effects on a wide variety of research endpoints.

  • Lower, more appropriate protein levels can improve survival and reduce morbidity
  • Vegetarian with no nitrosamines (a potential carcinogen)
  • Formulated to exclude alfalfa meal, greatly improving fluorescent imaging clarity
  • Formulated to exclude or lower soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Extruded rodent diets dramatically reduce clumping and hardness after autoclaving (2018SX,?2019S,?2020SX), and in general result in less diet waste and cleaner cages

Teklad rodent diets are natural-ingredient diets specifically formulated to provide the proper balance of all known nutrients considered essential for the growth, maintenance, and reproduction of rats, mice, gerbils and hamsters. These diets conform to the nutrient requirements for rodents established by the National Research Council (1995).

Teklad rodent diets provide uniform nutrition. They are fixed-formula diets designed to minimize the nutrient variances which otherwise could occur if the ingredient composition of a diet were altered from one batch to the next.

Protein is supplied primarily by plant sources. Supplemental amino acids are added to provide the proper amount and balance of essential amino acids. All rodent diets are fortified with vitamins and minerals to help support the regulation of body fluids and the proper functioning of body systems to ensure the adequate growth, maintenance, and reproduction of research rodents. Autoclavable diets are supplemented with additional vitamins to compensate for losses that occur during autoclaving. Since our diets are nutritionally complete and balanced, it is not necessary to add dietary supplements.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a six month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to nine months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least nine months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.

Recommended storage conditions:

  • Cool and dry; at or below 70 degrees fahrenheit with humidity ideally below 50%, but up to 65% is acceptable
  • Clean and free of pests
  • In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air

Standard natural ingredient rodent diets are complete diets for rats, mice, gerbils and hamsters, that should be allowed?ad libitum?access to the diet. Fresh water should be available at all times.

Harlan TD.88137 高酯飼料Adjusted calories diet (42% from fat)說明書

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Harlan TD.88137 高酯飼料Adjusted calories diet (42% from fat)說明書

Harlan Teklad代理，歡迎訪問Harlan Teklad官網(wǎng)或者咨詢我們獲取更多相關(guān)產(chǎn)品信息。

A staple of atherosclerosis research

More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe?deficient mouse model.

With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.

Formula g/Kg
Casein 195.0
DL-Methionine 3.0
Sucrose 341.46
Corn Starch 150.0
Andydrous Milkfat 210.0
Cholesterol 1.5
Cellulose 50.0
Mineral Mix, AIN-76 (170915) 35.0
Calcium Carbonate 4.0
Vitamin Mix, Teklad (40060) 10.0
Ethoxyquin 0.04

Selected nutrient information1

% By weight % kcal from
Protein 17.3 15.2
Carbohydrate 48.5 42.7
Fat 21.2 42.0
kcal/g 4.5
Cholesterol2 0.2%
  • Values are calculated from ingredient analysis or manufacturer data.
  • 15% added, 0.05% from fat source.

Critical dietary features of TD.88137 related to atherosclerosis development include:

++Cholesterol (0.2% total cholesterol)

++Total fat (21% by weight; 42% kcal from fat)

++High in saturated fatty acids

(>60% of total fatty acids)

++High sucrose (34% by weight)

Typical fatty acid profile of TD.88137

Typical fatty acid analysis, Mean SD
% of diet1
Total 20.7 1.5
Saturated fat 12.8 0.8
Monounsaturated fat 5.6 0.5
Polyunsaturated fat 1.0 0.2
Unknown2 1.3 0.3
Typical fatty acid profile, Mean SD
% of total fatty acids1
Saturated fat 61.8 2.0
Monounsaturated fat 27.3 2.1
Polyunsaturated fat 4.7 0.8
4:0 2.1 1.1
6:0 1.5 0.7
8:0 1.1 0.3
10:0 2.6 0.5
12:0 3.3 0.5
14:0 10.6 0.9
16:0 28.9 1.3
16:1 1.5 0.2
18:0 12.5 0.8
18:1 (Oleic) 20.9 2.6
18:1 Isomers3 4.0 1.2
18:2 (Linoleic) 2.3 1.0
18:2 Isomers4 1.3 0.5
18:3 (Linolenic) 0.7 0.2
  • n = 21, analysis conducted by two independent laboratories.
  • Unidentified fatty acids and those contributing on average less than 0.5% of total fatty acids.
  • Includes trans isomers elaidic and vaccenic acid and unidentified cis isomers.
  • Includes trans isomers.

Key points from the literature

TD.88137 has been used to accelerate atherosclerosis development in Apoe?and Ldlr?deficient models:

++In?Apoe?deficient mice, plasma cholesterol triples?to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks (2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).

++Ldlr?deficient mice fed for two weeks increase?plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic arch (6, 7).

For further information about TD.88137, or if you are interested in learning more about other atherogenic or high fat diets contact us at

With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis, hypertension and metabolic syndrome. Contact us for a more extensive reference list.

Control diet options for TD.88137

Natural ingredient diets

++Also referred to as standard diets or chow

++Diets differ in the source and level of nutrients?as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared to TD.88137

++Limits inferences to differences in dietary pattern?versus a specific dietary component

Ingredient matched, low fat diets

++Controls for the type of ingredients, non-nutritive?components and the source and level of specific nutrients

++Suggested ingredient matched, low fat dietary?controls for TD.88137 listed below; data sheets can be found on our website at

Suggested ingredient matched, low fat controls

Diet kcal/g Fat, % by % kcal Fat sources, Sucrose, %
weight from fat % by weight by weight
3.7% milk fat,
TD.05230 3.7 5.2 12.6 1.3% soybean 34.1
oil
3.7% milk fat,
TD.08485 3.6 5.2 13.0 1.3% soybean 12.0
oil

Additional controls are available. Contact a nutritionist at

Key planning information:

  • Store diet refrigerated and plan to use within six months. Diet should be replaced at minimum once per week when fed on cage tops.
  • Diets available as a soft ?” pellet or as a crumbly powder.
  • Three kg minimum order quantity. For planning purposes, estimates for diet uses (including feed intake and diet waste) are 5 g of diet per mouse and 30 g of diet per rat per day.
  • Two-day lead time for orders less than 10 kg. Two-week lead time for larger quantity orders.
  • Lead time for irradiation adds two weeks for any quantity of diet and must be requested at the time you place your order. Changes in texture and browning may occur with irradiation.
  • Shipping can affect pellet quality. Vacuum packaging can offer protection of the pellets during shipping. Two-day shipping is recommended during warmer months.

Contact us to place an order,

References

  1. Plump, A.S., et al.,Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell, 1992. 71(2): p. 343-53.
  2. Nakashima, Y., et al.,Apoe-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994.?14(1): p. 133-40.
  3. Febbraio, M., et al.,Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.

J Clin Invest, 2000. 105(8): p. 1049-56.

  1. Nakashima, Y., et al.,Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb

Vasc Biol, 1998. 18(5): p. 842-51.

  1. Towler, D.A., et al.,Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.

J Biol Chem, 1998. 273(46): p. 30427-34.

  1. Tsuchiya, K., et al.,FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
  2. Huszar, D., et al.,Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1. Arterioscler?Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
  3. Yang, B., et al.,Changes of skeletal muscle adiponectin content in diet-induced insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
  4. Schafer, K., et al.,Leptin promotes vascular remodeling and neointimal growth in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
  5. Lijnen, H.R., et al.,Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc?Biol, 2003. 23(1): p. 78-84.
  6. Maquoi, E., et al.,Modulation of adipose tissue expression of murine matrix metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002.?51(4): p. 1093-101.
  7. VanSaun MN, et al. High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol 175:355-64.
  8. Dixon LJ, et al. Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One 8:e56100.