標簽歸檔：nugen

Nugen 0340-32 人類FFPE RNA文庫構(gòu)建試劑盒在Illumina儀器平臺上測序的鏈特異性RNA-Seq文庫構(gòu)建提供了整體解決方案

發(fā)表于由

Ovation人類FFPE RNA-Seq多重文庫構(gòu)建試劑盒是為在Illumina儀器平臺上測序的鏈特異性RNA-Seq文庫構(gòu)建提供了整體解決方案。 該試劑盒結(jié)合了高效靈活的工作流程，適用于大范圍的總RNA輸入（10-250 ng），并采用AnyDeplete技術(shù)有效去除人類rRNA序列的垃圾背景序列。

Ovation人類FFPE RNA-Seq多重文庫構(gòu)建試劑盒使用從人類組織或細胞系獲得的納克級總RNA，為鏈特異性RNA-Seq文庫構(gòu)建提供了端到端的解決方案。該工作流程同樣適用于新鮮冷凍樣品（低至10 ng）的總RNA或FFPE樣品（低至100 ng）的保存RNA。從總RNA輸入到完整構(gòu)建的文庫的整合工作流程具有很高的可重復性，可以在大約7小時內(nèi)完成。 NuGEN的依賴插入的AnyDeplete技術(shù)可有針對性地消除不需要的轉(zhuǎn)錄本，并顯著減少了源自人類rRNA（12S，16S，18S和28S基因）的測序數(shù)據(jù)，從而更有效地利用了測序通量。該試劑盒均提供八個*的條形碼接頭，以使多重測序能夠進一步優(yōu)化功效。

簡化的工作流程包括使用隨機和多聚（T）引物的混合物反轉(zhuǎn)錄生成雙鏈cDNA，雙鏈cDNA打斷，末端修復以生成平末端，接頭進行連接，通過核苷酸類似物靶向降解的鏈選擇，AnyDeplete技術(shù)介導的接頭裂解和PCR擴增以產(chǎn)生終文庫。 整個工作流程，包括片段化，都可在短短7個小時內(nèi)完成，并生成可用于在Illumina測序平臺上進行單端或雙端測序的簇形成的DNA文庫。

NuGEN系列產(chǎn)品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation? SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation? SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation? SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation? SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation? RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation? RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation? RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation? RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation? Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation? Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation? Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl? oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl? oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl? oxBS , 32rxn
NuGEN #0414-32 TrueMethyl? oxBS Module , 32rxn
NuGEN #3302-12 Ovation? Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation? Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation? Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation? FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation? FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation? FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation? RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation? RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation? RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation? Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation? Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation? Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation? Ultralow System V2 , 96rxn

NuGEN 0506 Trio微量樣本全轉(zhuǎn)錄組文庫制備試劑盒

發(fā)表于由

Trio RNA-Seq文庫制備試劑盒提供同類更佳的NGS文庫制備系統(tǒng)和RNA測序解決方案，用于液體活檢，F(xiàn)FPE和其他微量的挑戰(zhàn)性樣品。

Trio微量RNA-Seq專為穩(wěn)定可靠的RNA-Seq文庫制備而設(shè)計，可與500 pg-50 ng的總RNA配合使用，可幫助研究人員從幾乎任何樣本中捕獲完整的生物學信息。

更新的Trio微量RNA-Seq文庫制備試劑盒現(xiàn)在集成了來自大量文獻發(fā)表的Ovation?RNA-Seq System V2和雙重index的成熟技術(shù)，可提供從低樣本量或低頻轉(zhuǎn)錄本的總RNA到完整文庫制備的準確流程。

NuGEN的雙重index可以方便地以預裝微孔板96個的條形碼對，因此無需手動準備條形碼對。只需將接頭添加到樣品中并連接，每個樣品將具有index1和index2條形碼，這些條形碼與平板中的所有其他樣品不同。

Trio微量RNA-Seq文庫制備結(jié)合了三種強大的技術(shù)：

1.單引物等溫擴增技術(shù)（SPIA）可以擴增微量的和降解的樣品。

2.?酶切片段化和DimerFree文庫構(gòu)建激素允許高效和穩(wěn)定的文庫制備。

3.在構(gòu)建文庫后，AnyDeplete技術(shù)可定制轉(zhuǎn)錄本耗盡方案，大化信息序列reads全轉(zhuǎn)錄組數(shù)據(jù)。

這種的工作流程為整個轉(zhuǎn)錄組文庫提供了無接頭二聚體和整合的無用轉(zhuǎn)錄本耗盡，從而增強了檢測和更均勻的轉(zhuǎn)錄本覆蓋率。

NuGEN系列產(chǎn)品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation? SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation? SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation? SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation? SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation? RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation? RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation? RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation? RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation? Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation? Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation? Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl? oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl? oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl? oxBS , 32rxn
NuGEN #0414-32 TrueMethyl? oxBS Module , 32rxn
NuGEN #3302-12 Ovation? Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation? Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation? Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation? FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation? FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation? FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation? RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation? RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation? RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation? Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation? Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation? Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation? Ultralow System V2 , 96rxn