Immobilon-PSQ膜是由聚偏氟乙烯(PVDF)制成的0.2 μm微孔膜。該產(chǎn)品是為了最大化蛋白質(zhì)結(jié)合而開(kāi)發(fā)的。Immobilon-PSQ膜推薦用于電泳凝膠電泳蛋白(或純化蛋白樣品的點(diǎn)結(jié)合)后的直接蛋白測(cè)序和免疫檢測(cè),特別是分子量小于20,000的蛋白。該濾膜兼容儲(chǔ)水罐和半干法電印跡系統(tǒng)。
應(yīng)用
Immobilon-PSQ PVDF膜已用于蛋白質(zhì)印跡分析。
特點(diǎn)和優(yōu)勢(shì)
Immobilon-PSQ膜:
0.2 μm孔徑和大內(nèi)部結(jié)構(gòu)
蛋白質(zhì)吸附和測(cè)序產(chǎn)量高于其他膜
防止低分子量蛋白質(zhì)的吹穿
物料
PVDF membrane
plain filter
white filter
質(zhì)量水平
400
特點(diǎn)
hydrophobic
manufacturer/tradename
Immobilon
technique(s)
western blot: suitable
過(guò)濾器長(zhǎng)度 × 寬度
26.5cm × 3.75m
孔徑
0.2μm pore size
容量
262μg/cm2 adsorption capacity (insulin)
340μg/cm2 adsorption capacity (BSA)
448μg/cm2 adsorption capacity (goat IgG)
相容性
for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro ruby
for use with Toluidine blue
for use with Transillumination
detection method
chemiluminescent
colorimetric
radioactive