The NxSeq UltraLow DNA Library Kits v2 and NxSeq Indexing Kits are only compatible with Illumina sequencers.

The NxSeq UltraLow DNA Library Kits contain Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG), 2X PCR Master Mix v2 (MM) and Elution Buffer (EB). For optimal performance, we recommend pairing our NxSeq UltraLow Kits with the NxSeq Indexing Kits. Contact us if you need more or different style indexes.

Each NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. Each NxSeq Single Index equals the TruSeq® LT Index with the same number.

The NxSeq HT Dual Indexing Kit contains a Universal Adaptor, Adaptor Dilution Buffer and Indexing Primers 701 – 712, and 501 – 508. There is enough of each reagent in this kit to generate 96 different combinatorial dual indexed libraries. Each NxSeq HT Dual Index equals the TruSeq® HT Index with the same number.

• High Quality Data: High efficiency adaptor ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer missed regions
• Sensitive: Construct DNA fragment libraries from as little as 50 pg to as much as 100 ng of sheared/fragmented DNA
• Minimal Bias: Better adaptor ligation efficiency and more uniform PCR amplification improves coverage uniformity when working with normal and low input amounts of genomic DNA
• Flexible: Compatible with multiple applications including whole genome sequencing or resequencing, exome-seq, ChIP-seq and FFPE/cell-free DNA samples.
• Fast: 3 hour protocol gets your samples on the sequencer quicker
• High Value: Cost-effective library and indexing kits with excellent sequencing results