Lucigen | LGC代理,FailSafe Enzyme Mix,部分現(xiàn)貨


FailSafe Enzyme Mix

PCR Enzyme Mix, individual component of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post – optimization FailSafe PCR System.

Product Details

The PCR Enzyme Mix is a blend of thermostable DNA polymerases containing a 3′→5′ proofreading enzyme for high fidelity. Supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.5% Tween®20, 0.5% NP40, and 1 mM dithiothreitol.

Choose from 2 different sizes of FailSafe PCR Enzyme Mix, 100 Units (40 uL) and 1000 Units (400 uL).

Before purchasing we strongly recommend first optimising your PCR conditions using the FailSafe PCR PreMix Selection Kit. If optimization is complete, you can proceed with the bundled and affordable post optimisation FailSafe PCR System.

The “failsafe” nature is guaranteed when using all of the FailSafe components.

Key features

  • Unique blend of thermostable enzymes
  • 3′→5′ proofreading enzyme
  • High fidelity at least 3 times higher than Taq DNA polymerase

Select a Size

Please select a Size

Lucigen | LGC, Biosearch Technologies主要開發(fā)各類用于基因克隆的試劑盒及相關(guān)產(chǎn)品,包括:CloneSmart?平端克隆試劑盒、BigEasy?線性克隆系統(tǒng)、pEZSeq™平端克隆試劑盒、聚合酶及E.cloni?感受態(tài)細胞及蛋白表達系統(tǒng)等。

Lucigen公司憑借其獨到的產(chǎn)品技術(shù),過硬的產(chǎn)品質(zhì)量,良好的產(chǎn)品服務(wù)贏得了全球廣大用戶的信賴。此外,Lucigen還是全球科學(xué)家青睞的Epicentre品牌產(chǎn)品的供應(yīng)商。在2018年,Lucigen被LGC收購,從而加強了其在基因組市場的試劑供應(yīng)。Lucigen的產(chǎn)品和服務(wù)已經(jīng)覆蓋了臨床診斷、藥物開發(fā)、合成生物學(xué)和基因編輯等多個領(lǐng)域。更多產(chǎn)品信息歡迎訪問Lucigen | LGC, Biosearch Technologies官網(wǎng)。

Lucigen | LGC代理,LavaLAMP DNA Master Mix,部分現(xiàn)貨


LavaLAMP DNA Master Mix

Sensitive and Fast DNA LAMP in an easy-to-use Master Mix format.

Product Details

Both LavaLAMP DNA Master Mix kits contain: LavaLAMP DNA Master Mix, DNA Positive Control LAMP Primer Mix, and DNA Positive Control. The LavaLAMP DNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA.

Key features

  • Loop-mediated isothermal amplification (LAMP): Facilitates running amplification reactions in difficult test environments, enabling use outside of a high-tech laboratory.
  • Master Mix Format: Simplifies reaction setup and minimises optimisation required to produce the best assay results.
  • High Thermal Stability: Highly thermostable enzyme (up to 90 °C for 5 min) enables preheating of reactions containing purified target DNA which, depending on the target, may increase assay sensitivity and decrease time to results.
  • Elevated LAMP Reaction Temperature (68-74 °C):Improves primer specificity and reduces background amplification depending on the DNA target.
  • Freeze-dry Compatible: Enables generation of room temperature stable test kits through lyophilisation.

Select a Format

Please select a Format

Lucigen | LGC, Biosearch Technologies主要開發(fā)各類用于基因克隆的試劑盒及相關(guān)產(chǎn)品,包括:CloneSmart?平端克隆試劑盒、BigEasy?線性克隆系統(tǒng)、pEZSeq™平端克隆試劑盒、聚合酶及E.cloni?感受態(tài)細胞及蛋白表達系統(tǒng)等。

Lucigen公司憑借其獨到的產(chǎn)品技術(shù),過硬的產(chǎn)品質(zhì)量,良好的產(chǎn)品服務(wù)贏得了全球廣大用戶的信賴。此外,Lucigen還是全球科學(xué)家青睞的Epicentre品牌產(chǎn)品的供應(yīng)商。在2018年,Lucigen被LGC收購,從而加強了其在基因組市場的試劑供應(yīng)。Lucigen的產(chǎn)品和服務(wù)已經(jīng)覆蓋了臨床診斷、藥物開發(fā)、合成生物學(xué)和基因編輯等多個領(lǐng)域。更多產(chǎn)品信息歡迎訪問Lucigen | LGC, Biosearch Technologies官網(wǎng)。

Lucigen | LGC代理,LavaLAMP RNA Master Mix,部分現(xiàn)貨


LavaLAMP RNA Master Mix

Sensitive RNA detection with this easy-to-use RNA LAMP (RT-LAMP) Master Mix.

Product Details

Both LavaLAMP RNA Master Mix kits contain: LavaLAMP RNA Master Mix, RNA Positive Control LAMP Primer Mix, and RNA Positive Control. The LavaLAMP RNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.

Key features

  • Isothermal Amplification: Facilitates running amplification reactions with simplified instrumentation
  • Master Mix Format: Streamlines reaction setup while reducing potential handling errors
  • Minimal Optimisation: Focuses optimisation on the two critical reaction parameters – primer design and reaction temperature

  • Lyophilisation-ready: Avoids redesign of assays to remove components known to inhibit lyophilisation all components are lyophilisation-compatible

Select a Format

Please select a Format

Lucigen | LGC, Biosearch Technologies主要開發(fā)各類用于基因克隆的試劑盒及相關(guān)產(chǎn)品,包括:CloneSmart?平端克隆試劑盒、BigEasy?線性克隆系統(tǒng)、pEZSeq™平端克隆試劑盒、聚合酶及E.cloni?感受態(tài)細胞及蛋白表達系統(tǒng)等。

Lucigen公司憑借其獨到的產(chǎn)品技術(shù),過硬的產(chǎn)品質(zhì)量,良好的產(chǎn)品服務(wù)贏得了全球廣大用戶的信賴。此外,Lucigen還是全球科學(xué)家青睞的Epicentre品牌產(chǎn)品的供應(yīng)商。在2018年,Lucigen被LGC收購,從而加強了其在基因組市場的試劑供應(yīng)。Lucigen的產(chǎn)品和服務(wù)已經(jīng)覆蓋了臨床診斷、藥物開發(fā)、合成生物學(xué)和基因編輯等多個領(lǐng)域。更多產(chǎn)品信息歡迎訪問Lucigen | LGC, Biosearch Technologies官網(wǎng)。

RT-PCR Mix for qPCR(預(yù)混型逆轉(zhuǎn)錄試劑盒)

RT-PCR Mix for qPCR(預(yù)混型逆轉(zhuǎn)錄試劑盒)

貨號:R1031   規(guī)格:100次(20 μl反應(yīng)體系)

 

產(chǎn)品說明

RT-PCR Mix for qPCR是預(yù)混型逆轉(zhuǎn)錄反應(yīng)試劑盒,操作簡便,可快速高效完成逆轉(zhuǎn)錄反應(yīng)。其中Enzyme Mix含有高效逆轉(zhuǎn)錄酶和熱敏型DNase,一步完成基因組DNA的清除以及RNA的逆轉(zhuǎn)錄反應(yīng),RT Buffer包含逆轉(zhuǎn)錄反應(yīng)所需的其他組分,無需分步多次加樣,降低了樣品污染的風險。同時,本試劑盒配備了Control Mix,用于陰性對照反應(yīng),檢驗RNA模板中是否有基因組DNA殘留。本試劑盒的逆轉(zhuǎn)錄產(chǎn)物對染料法和探針法熒光定量PCR反應(yīng)均有很好的兼容性,可進行精準的基因表達分析。

 

產(chǎn)品組分

組分

R1031100次)

Enzyme   Mix

100   μl

5X   RT Buffer

400 μl

Control   Mix

20   μl

RNase-free   ddH2O

1   ml × 2

 

保存條件

各組分均-20℃保存,并避免反復(fù)凍融。

 

適用范圍

第一鏈cDNA 合成

 

注意事項

本品含有熱敏型DNase,請于冰上使用本品各組分。

為保證成分均勻,請將Enzyme Mix、5X RT BufferControl Mix分別瞬時離心、吹打混勻后再吸取使用。

在操作過程中,注意防止引入RNase導(dǎo)致RNA降解。RNA最好保存于-70℃,避免反復(fù)凍融。

l cDNA產(chǎn)物應(yīng)置于-20℃或以下保存,避免反復(fù)凍融。

 

操作步驟

1. 配制反應(yīng)體系

按以下體系,在冰浴的無菌無酶離心管中配制反應(yīng)混合液:

組分

用量

5X   RT Buffer

4 μl

Enzyme   Mix

1 μl

RNA

1pg-1μg

RNase-free   ddH2O

補足至20 μl 

 

陰性對照反應(yīng)(可選)

即不加逆轉(zhuǎn)錄酶的反應(yīng),產(chǎn)物用于在qPCR中作為陰性對照,檢驗?zāi)0?span>RNA中是否有基因組DNA殘留

按以下體系,在冰浴的無菌無酶離心管中配制反應(yīng)混合液:

組分

用量

5X   RT Buffer  

4 μl

Control   Mix

1 μl

RNA

1pg-1μg

RNase-free   ddH2O

補足至20 μl 

 

2. 進行逆轉(zhuǎn)錄反應(yīng)

將上述混合液吹打混勻,瞬時離心收集至管底。按以下程序,在PCR儀中進行反應(yīng):

溫度

時間

50

15   min

85

5   sec

 

3. qPCR反應(yīng)及cDNA保存

cDNA產(chǎn)物可稀釋后立即進行染料法或探針法qPCR反應(yīng)。若使用未稀釋的cDNA原液,則體積不能超過反應(yīng)體系的1/10,以避免抑制反應(yīng)效率。長期保存cDNA產(chǎn)物應(yīng)于-20℃或以下環(huán)境中,并避免反復(fù)凍融。