HA&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)

產(chǎn)品編號(hào)

JP07045S
HA&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)
各1μg

1776

JP07045M
HA&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)
各100μg

2338

自行研發(fā)生產(chǎn)的HA&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)(Positive Control Plasmids Pair for HA&Myc Co-IP)包含pCMV-HA-JP5和pCMV-Myc-LTA兩種質(zhì)粒,可以用于在哺乳動(dòng)物細(xì)胞中表達(dá)N端帶有HA標(biāo)簽的人源JP5和Myc標(biāo)簽的LTA (large T antigen)融合蛋白。因?yàn)镴P5和LTA蛋白間具有較強(qiáng)的相互作用,本質(zhì)粒對(duì)可作為共轉(zhuǎn)染細(xì)胞免疫共沉淀(Co-Immunoprecipitation, Co-IP)的陽(yáng)性對(duì)照使用,也可以單獨(dú)轉(zhuǎn)染細(xì)胞作為免疫沉淀(Immunoprecipitation, IP)的陽(yáng)性對(duì)照使用。
免疫沉淀或免疫共沉淀是研究蛋白或蛋白與蛋白相互作用(Protein-Protein Interactions, PPIs)的常用實(shí)驗(yàn)技術(shù),通過(guò)使用特異性抗體和可結(jié)合抗體的介質(zhì)(如Protein A/G Agarose或Protein A/G磁珠),或直接使用偶聯(lián)特異性抗體的介質(zhì)(如瓊脂糖凝膠或磁珠),然后通過(guò)離心或磁力從溶液中分離出抗原和抗體復(fù)合物,從而將目標(biāo)蛋白質(zhì)從復(fù)雜樣品中分離出來(lái),隨后可以用于Western印跡檢測(cè)或質(zhì)譜分析等[1-2]。
pCMV-HA-JP5質(zhì)粒表達(dá)JP5。JP5中文名為細(xì)胞腫瘤抗原JP5 (Cellular tumor antigen JP5),全長(zhǎng)393氨基酸,理論分子量為43.7kDa,實(shí)際分子量約為53kDa,是調(diào)控細(xì)胞周期的重要轉(zhuǎn)錄因子,主要功能包括調(diào)節(jié)細(xì)胞周期、促進(jìn)細(xì)胞凋亡、維持基因組穩(wěn)定性和抑制腫瘤血管生成等,野生型的JP5是一個(gè)重要的抑癌基因[3]。
pCMV-Myc-LTA質(zhì)粒表達(dá)LTA。LTA即SV40大T抗原(SV40 large T antigen),也稱(chēng)SV40gp6 large T antigen (Macaca mulatta polyomavirus 1),全長(zhǎng)708氨基酸,分子量為81.6kDa,是一種六聚體蛋白。當(dāng)LTA單獨(dú)表達(dá)時(shí)能誘導(dǎo)靜止細(xì)胞的增殖和轉(zhuǎn)化,可以誘導(dǎo)實(shí)驗(yàn)動(dòng)物腫瘤的形成,為研究DNA復(fù)制和細(xì)胞轉(zhuǎn)化提供了非常有用的模型[4]。
本質(zhì)粒對(duì)均含有CMV啟動(dòng)子,可以高效啟動(dòng)目的蛋白在細(xì)胞中的表達(dá),均帶有氨芐青霉素(Ampicillin)抗性和新霉素(Neomycin)抗性。
本質(zhì)粒對(duì)用于磁珠法免疫共沉淀的效果參考圖1A,用于瓊脂糖凝膠法免疫共沉淀的效果參考圖1B。

圖1. 的HA&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)(JP07045)用于免疫共沉淀的效果圖。圖A使用了HA標(biāo)簽蛋白免疫沉淀試劑盒(磁珠法) (JP2085)和Myc標(biāo)簽蛋白免疫沉淀試劑盒(磁珠法) (JP2083),圖B使用了HA標(biāo)簽蛋白免疫沉淀試劑盒(瓊脂糖凝膠法) (JP2006)和Myc標(biāo)簽蛋白免疫沉淀試劑盒(瓊脂糖凝膠法) (JP2004)。293T細(xì)胞(人胚腎細(xì)胞)單獨(dú)或共轉(zhuǎn)染pCMV-HA-JP5和pCMV-Myc-LTA質(zhì)粒36小時(shí)后,使用Western及IP細(xì)胞裂解液(JP010013)裂解。泳道1為小鼠IgG磁珠或瓊脂糖凝膠免疫沉淀后洗脫的樣品,為免疫共沉淀的陰性對(duì)照;泳道2、3和4為磁珠或瓊脂糖凝膠免疫沉淀后洗脫的樣品,其中圖A是經(jīng)HA Peptide或c-Myc Peptide洗脫后的樣品,圖B是經(jīng)SDS-PAGE Sample Loading Buffer洗脫后的樣品,從泳道4可觀察到共轉(zhuǎn)染的HA-JP5與Myc-LTA可以相互作用。Input即全細(xì)胞裂解液(Total cell lysate)。Western印跡成像由BeyoImager? 600化學(xué)發(fā)光成像系統(tǒng)完成(EI600)。實(shí)際結(jié)果會(huì)因?qū)嶒?yàn)條件、檢測(cè)儀器等的不同而存在差異,圖中數(shù)據(jù)僅供參考。
推薦使用的測(cè)序引物序列如下:
CMV-F primer: 5′ -CGCAAATGGGCGGTAGGCGTG-3′
BGH-R primer: 5′-TAGAAGGCACAGTCGAGG-3′
pCMV-HA-JP5和pCMV-Myc-LTA的全序列信息:JP07045-1 pCMV-HA-JP5-全序列.txt;JP07045-2 pCMV-Myc-LTA-全序列.txt。
包裝清單:

產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
包裝

JP07045S-1
pCMV-HA-JP5
1μg

JP07045S-2
pCMV-Myc-LTA
1μg


說(shuō)明書(shū)
1份

產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
包裝

JP07045M-1
pCMV-HA-JP5
100μg

JP07045M-2
pCMV-Myc-LTA
100μg


說(shuō)明書(shū)
1份

保存條件:
-20℃保存。
注意事項(xiàng):
本質(zhì)粒未經(jīng)書(shū)面許可不得用于任何商業(yè)用途,也不得移交給訂貨人所在實(shí)驗(yàn)室外的任何個(gè)人或單位。
本質(zhì)粒對(duì)僅經(jīng)過(guò)測(cè)序、免疫沉淀和免疫共沉淀驗(yàn)證,未經(jīng)過(guò)功能性驗(yàn)證。
本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Flag&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)

產(chǎn)品編號(hào)

JP07041S
Flag&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)
各1μg

1776

JP07041M
Flag&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)
各100μg

2338

自行研發(fā)生產(chǎn)的Flag&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)(Positive Control Plasmids Pair for Flag&Myc Co-IP)包含pCMV-3X Flag-JP5和pCMV-Myc-LTA兩種質(zhì)粒,可以用于在哺乳動(dòng)物細(xì)胞中表達(dá)N端帶有3X Flag標(biāo)簽的人源JP5和Myc標(biāo)簽的LTA (large T antigen)融合蛋白。因?yàn)镴P5和LTA蛋白間具有較強(qiáng)的相互作用,本質(zhì)粒對(duì)可作為共轉(zhuǎn)染細(xì)胞免疫共沉淀(Co-Immunoprecipitation, Co-IP)的陽(yáng)性對(duì)照使用,也可以單獨(dú)轉(zhuǎn)染細(xì)胞作為免疫沉淀(Immunoprecipitation, IP)的陽(yáng)性對(duì)照使用。
免疫沉淀或免疫共沉淀是研究蛋白或蛋白與蛋白相互作用(Protein-Protein Interactions, PPIs)的常用實(shí)驗(yàn)技術(shù),通過(guò)使用特異性抗體和可結(jié)合抗體的介質(zhì)(如Protein A/G Agarose或Protein A/G磁珠),或直接使用偶聯(lián)特異性抗體的介質(zhì)(如瓊脂糖凝膠或磁珠),然后通過(guò)離心或磁力從溶液中分離出抗原和抗體復(fù)合物,從而將目標(biāo)蛋白質(zhì)從復(fù)雜樣品中分離出來(lái),隨后可以用于Western印跡檢測(cè)或質(zhì)譜分析等[1-2]。
pCMV-3X Flag-JP5質(zhì)粒表達(dá)JP5。JP5中文名為細(xì)胞腫瘤抗原JP5 (Cellular tumor antigen JP5),全長(zhǎng)393氨基酸,理論分子量為43.7kDa,實(shí)際分子量約為53kDa,是調(diào)控細(xì)胞周期的重要轉(zhuǎn)錄因子,主要功能包括調(diào)節(jié)細(xì)胞周期、促進(jìn)細(xì)胞凋亡、維持基因組穩(wěn)定性和抑制腫瘤血管生成等,野生型的JP5是一個(gè)重要的抑癌基因[3]。
pCMV-Myc-LTA質(zhì)粒表達(dá)LTA。LTA即SV40大T抗原(SV40 large T antigen),也稱(chēng)SV40gp6 large T antigen (Macaca mulatta polyomavirus 1),全長(zhǎng)708氨基酸,分子量為81.6kDa,是一種六聚體蛋白。當(dāng)LTA單獨(dú)表達(dá)時(shí)能誘導(dǎo)靜止細(xì)胞的增殖和轉(zhuǎn)化,可以誘導(dǎo)實(shí)驗(yàn)動(dòng)物腫瘤的形成,為研究DNA復(fù)制和細(xì)胞轉(zhuǎn)化提供了非常有用的模型[4]。
本質(zhì)粒對(duì)均含有CMV啟動(dòng)子,可以高效啟動(dòng)目的蛋白在細(xì)胞中的表達(dá),均帶有氨芐青霉素(Ampicillin)抗性和新霉素(Neomycin)抗性。
本質(zhì)粒對(duì)用于磁珠法免疫共沉淀的效果參考圖1A,用于瓊脂糖凝膠法免疫共沉淀的效果參考圖1B。

圖1. 的Flag&Myc Co-IP陽(yáng)性對(duì)照質(zhì)粒對(duì)(JP07041)用于免疫共沉淀的效果圖。圖A使用了Flag標(biāo)簽蛋白免疫沉淀試劑盒(磁珠法) (JP2081)和Myc標(biāo)簽蛋白免疫沉淀試劑盒(磁珠法) (JP2083),圖B使用了Flag標(biāo)簽蛋白免疫沉淀試劑盒(瓊脂糖凝膠法) (JP2002)和Myc標(biāo)簽蛋白免疫沉淀試劑盒(瓊脂糖凝膠法) (JP2004)。293T細(xì)胞(人胚腎細(xì)胞)單獨(dú)或共轉(zhuǎn)染pCMV-3X Flag-JP5和pCMV-Myc-LTA質(zhì)粒36小時(shí)后,使用Western及IP細(xì)胞裂解液(JP010013)裂解,并使用相應(yīng)的標(biāo)簽蛋白免疫沉淀試劑盒進(jìn)行免疫沉淀。泳道1為小鼠IgG磁珠或瓊脂糖凝膠免疫沉淀后洗脫的樣品,為免疫共沉淀的陰性對(duì)照;泳道2、3和4為磁珠或瓊脂糖凝膠免疫沉淀后洗脫的樣品,其中圖A是經(jīng)3X Flag Peptide或c-Myc Peptide洗脫后的樣品,圖B是經(jīng)SDS-PAGE Sample Loading Buffer洗脫后的樣品,從泳道4可觀察到共轉(zhuǎn)染的Flag-JP5與Myc-LTA可以相互作用。Input即全細(xì)胞裂解液(Total cell lysate)。Western印跡成像由BeyoImager? 600化學(xué)發(fā)光成像系統(tǒng)完成(EI600)。實(shí)際結(jié)果會(huì)因?qū)嶒?yàn)條件、檢測(cè)儀器等的不同而存在差異,圖中數(shù)據(jù)僅供參考。
推薦使用的測(cè)序引物序列如下:
CMV-F primer: 5′ -CGCAAATGGGCGGTAGGCGTG-3′
BGH-R primer: 5′-TAGAAGGCACAGTCGAGG-3′
pCMV-3X Flag-JP5和pCMV-Myc-LTA的全序列信息:JP07041-1 pCMV-3X Flag-JP5-全長(zhǎng)序列.txt;JP07041-2 pCMV-Myc-LTA-全序列.txt。
包裝清單:

產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
包裝

JP07041S-1
pCMV-3X Flag-JP5
1μg

JP07041S-2
pCMV-Myc-LTA
1μg


說(shuō)明書(shū)
1份

產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
包裝

JP07041M-1
pCMV-3X Flag-JP5
100μg

JP07041M-2
pCMV-Myc-LTA
100μg


說(shuō)明書(shū)
1份

保存條件:
-20℃保存。
注意事項(xiàng):
本質(zhì)粒未經(jīng)書(shū)面許可不得用于任何商業(yè)用途,也不得移交給訂貨人所在實(shí)驗(yàn)室外的任何個(gè)人或單位。
本質(zhì)粒對(duì)僅經(jīng)過(guò)測(cè)序、免疫沉淀和免疫共沉淀驗(yàn)證,未經(jīng)過(guò)功能性驗(yàn)證。
本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Mouse Anti-Myc Monoclonal Antibody(9E10)

Mouse Anti-Myc Monoclonal Antibody(9E10) 

貨號(hào):NBK1501

規(guī)格:30ul / 100ul / 1ml

品牌:JinPan

 Product Data
Product Name Mouse Anti-Myc Monoclonal Antibody(9E10) 
Clone Name 9E10
Applications FC, IF, IP, WB
Target Myc tag
Host Mouse
Clonality Monoclonal
Immunogen AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of human c-Myc
Formulation 0.64 mg/ml in PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration Lot dependent
Conjugation Unconjugated
Storage Condition Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Synonyms 9E10 antibody; 9E10 anti myc; 9E10 myc; anti-Myc; anti-myc antibodies; Anti-myc Antibody; anti c-myc; anti cmyc; anti myc; c-myc antibody; cmyc antibody; myc antibody; tag antibody

9E10, Anti-Myc Monoclonal Antibody9E10, 抗真菌單克隆抗體

 Product Data
Product Name 9E10, Anti-Myc Monoclonal Antibody
Clone Name 9E10
Applications FC, IF, IP, WB
Target Myc tag
Host Mouse
Clonality Monoclonal
Immunogen AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of human c-Myc
Isotype IgG1
Formulation 0.64 mg/ml in PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration Lot dependent
Conjugation Unconjugated
Storage Condition Store at -20°C.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation ,Wang, W;Qin, Y;Song, H;Wang, L;Jia, M;Zhao, C;Gong, M;Zhao, W;, Journal of immunology (Baltimore, Md. : 1950) 2021 ,PubMed ID 33963043 [MYC]
USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS ,Hou, J;Han, L;Zhao, Z;Liu, H;Zhang, L;Ma, C;Yi, F;Liu, B;Zheng, Y;Gao, C;, Nature communications 2021 ,PubMed ID 34016972 [MYC]
Phosphatase Shp2 regulates biogenesis of small extracellular vesicles by dephosphorylating Syntenin ,Zhang, Y;Li, Y;Liu, P;Gong, D;Zhou, H;Li, W;Zhang, H;Zheng, W;Xu, J;Cheng, H;Zhang, X;Ke, Y;, Journal of extracellular vesicles 2021 ,PubMed ID 33732417 [MYC]
Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1 ,Li, X;Li, S;Li, B;Li, Y;Aman, S;Xia, K;Yang, Y;Ahmad, B;Wu, H;, NPJ precision oncology 2021 ,PubMed ID 33742100 [MYC]
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) membrane (M) protein inhibits type I and III interferon production by targeting RIG-I/MDA-5 signaling ,Zheng, Y;Zhuang, M;Han, L;Zhang, J;Nan, M;Zhan, P;Kang, D;Liu, X;Gao, C;Wang, P;, Signal Transduction and Targeted Therapy 2020 ,PubMed ID 33372174 [MYC]
Suppression of Membranous LRP5 Recycling, Wnt/β-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P ,Liu, F;Zuo, X;Liu, Y;Deguchi, Y;Moussalli, MJ;Chen, W;Yang, P;Wei, B;Tan, L;Lorenzi, PL;Gao, S;Jaoude, JC;Mehdizadeh, A;Valentin, LA;Wei, D;Shureiqi, I;, Cell Rep 2020 ,PubMed ID 32814052 [MYC]
TAOK1 positively regulates TLR4-induced inflammatory responses by promoting ERK1/2 activation in macrophages ,Zhu, L;Yu, Q;Gao, P;Liu, Q;Luo, X;Jiang, G;Ji, R;Yang, R;Ma, X;Xu, J;Yuan, H;Zhou, J;An, H;, Mol. Immunol. 2020 ,PubMed ID 32344244 [MYC]
Downregulation of hippocampal SIRT6 activates AKT/CRMP2 signaling and ameliorates chronic stress-induced depression-like behavior in mice ,Li, W;Liu, X;Qiao, H;, Acta Pharmacol. Sin. 2020 ,PubMed ID 32265492 [MYC]
Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3 ,Thayyullathil, F;Cheratta, A;Pallichankandy, S;Subburayan, K;Tariq, S;Rangnekar, V;Galadari, S;, Biochimica et Biophysica Acta (BBA) – Molecular Cell Research 2020 ,PubMed ID 32135176 [MYC]
The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context ,Schüchner, S;Behm, C;Mudrak, I;Ogris, E;, Sci Signal 2020 ,PubMed ID 31992583 [MYC]
Ku80 negatively regulates the expression of OCT4 via competitive binding to SALL4 and promoting lysosomal degradation of OCT4 ,Zhao, B;Zheng, X;Tan, X;Ke, K;Wang, F;Wang, Y;Xing, X;Zhang, C;Hu, P;Lan, S;Li, Q;Huang, A;Liu, X;, Int. J. Biochem. Cell Biol. 2019 ,PubMed ID 31816404 [MYC]
NOD1 inhibits proliferation and enhances response to chemotherapy via suppressing SRC-MAPK pathway in hepatocellular carcinoma ,Ma, X;Qiu, Y;Zhu, L;Zhao, Y;Lin, Y;Ma, D;Qin, Z;Sun, C;Shen, X;Li, T;Han, L;, J. Mol. Med. 2019 ,PubMed ID 31872284 [MYC]
Mink Enteritis Virus Infection Induces Mitochondrion-mediated Apoptosis by the Viral Nonstructural Protein 1 ,Lin, P;Cheng, Y;Song, S;Qiu, J;Yi, L;Cao, Z;Li, J;Cheng, S;Wang, J;, J. Virol. 2019 ,PubMed ID 31484746 [MYC]
Poly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or α-synuclein, is localized to the mitochondrial matrix ,Osuagwu, N;D?lle, C;Tzoulis, C;, PLoS ONE 2019 ,PubMed ID 31323073 [MYC]
Regulation of dendrite morphology and excitatory synapse formation by zDHHC15 ,Shah, BS;Shimell, JJ;Bamji, SX;, J. Cell. Sci. 2019 ,PubMed ID 31189538 [MYC]
Aquaporin-4 Surface Trafficking Regulates Astrocytic Process Motility and Synaptic Activity in Health and Autoimmune Disease ,Ciappelloni, S;Bouchet, D;Dubourdieu, N;Boué-Grabot, E;Kellermayer, B;Manso, C;Marignier, R;Oliet, SHR;Tourdias, T;Groc, L;, Cell Rep 2019 ,PubMed ID 31242419 [MYC]
TRIM50 acts as a novel Src suppressor and inhibits ovarian cancer progression ,Qiu, Y;Liu, P;Ma, X;Ma, X;Zhu, L;Lin, Y;You, Y;Yu, W;Ma, D;Sun, C;Qin, Z;Zhao, Y;Shi, J;Han, L;, Biochim Biophys Acta Mol Cell Res 2019 ,PubMed ID 31176697 [MYC]
Membrane metalloprotease TRABD2A restricts HIV-1 progeny production in resting CD4+ T cells by degrading viral Gag polyprotein ,Liang, G;Zhao, L;Qiao, Y;Geng, W;Zhang, X;Liu, M;Dong, J;Ding, H;Sun, H;Shang, H;, Nat. Immunol. 2019 ,PubMed ID 31061530 [MYC]
USP19 Inhibits TNF-α- and IL-1β-Triggered NF-κB Activation by Deubiquitinating TAK1 ,Lei, CQ;Wu, X;Zhong, X;Jiang, L;Zhong, B;Shu, HB;, J. Immunol. 2019 ,PubMed ID 31127032 [MYC]
Glia maturation factor-γ regulates murine macrophage iron metabolism and M2 polarization through mitochondrial ROS ,Aerbajinai, W;Ghosh, MC;Liu, J;Kumkhaek, C;Zhu, J;Chin, K;Rouault, TA;Rodgers, GP;, Blood Adv 2019 ,PubMed ID 30971398 [MYC]
Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats ,Li, X;Jin, X;Sharma, S;Liu, X;Zhang, J;Niu, Y;Li, J;Li, Z;, BioRxiv 2019 [MYC]
Escherichia coli as a platform for the study of phosphoinositide biology ,Botero, S;Chiaroni-Clarke, R;Simon, SM;, Sci Adv 2019 ,PubMed ID 30944849 [MYC]
Phosphatase Shp2 exacerbates intestinal inflammation by disrupting macrophage responsiveness to interleukin-10 ,Xiao, P;Zhang, H;Zhang, Y;Zheng, M;Liu, R;Zhao, Y;Zhang, X;Cheng, H;Cao, Q;Ke, Y;, J. Exp. Med. 2019 ,PubMed ID 30610104 [MYC]
Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival ,Losada, A;Mu?oz-Alonso, MJ;Martínez-Díez, M;Gago, F;Domínguez, JM;Martínez-Leal, JF;Galmarini, CM;, Br. J. Cancer 2018 ,PubMed ID 30420615 [MYC]
Combined effects of FH (E404D) and ACOX2 (R409H) cause metabolic defects in primary cardiac malignant tumor ,Zhou, X;Xu, M;Zeng, W;Chen, Z;Lu, G;Gong, Y;Finnell, RH;Xiao, H;Qiao, B;Wang, H;, Cell Death Discov 2018 ,PubMed ID 30062063 [MYC]
UBXN3B positively regulates STING-mediated antiviral immune responses ,Yang, L;Wang, L;Ketkar, H;Ma, J;Yang, G;Cui, S;Geng, T;Mordue, DG;Fujimoto, T;Cheng, G;You, F;Lin, R;Fikrig, E;Wang, P;, Nat Commun 2018 ,PubMed ID 29899553 [MYC]
TRIM50 suppressed hepatocarcinoma progression through directly targeting SNAIL for ubiquitous degradation ,Ma, X;Ma, X;Qiu, Y;Zhu, L;Lin, Y;You, Y;Ma, D;Qin, Z;Sun, C;Zhao, Y;Sun, Y;Han, L;, Cell Death Dis 2018 ,PubMed ID 29789583 [MYC]
Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer ,Totland, C;Kr?kenes, T;Mazengia, K;Haugen, M;Vedeler, C;, Oncotarget 2018 ,PubMed ID 29844866 [MYC]
Porcine enterocyte protein Btnl5 negatively regulates NF-kappa B pathway by interfering p65 nuclear translocation ,Bao, Q;Li, C;Xu, C;Zhang, R;Zhao, K;Duan, Z;, Gene 2017 ,PubMed ID 29197592 [Myc]
Intracellular mature IL-37 suppresses tumor metastasis via inhibiting Rac1 activation ,Li, Y;Zhao, M;Guo, C;Chu, H;Li, W;Chen, X;Wang, X;Li, Y;Jia, Y;Koussatidjoa, S;Zhu, F;Wang, J;Wang, X;Wang, Q;Zhao, W;Shi, Y;Chen, W;Zhang, L;, Oncogene 2017 ,PubMed ID 29106392 [Myc]
25-Hydroxycholesterol Inhibition of Lassa Virus Infection through Aberrant GP1 Glycosylation ,Shrivastava-Ranjan, P;Bergeron, é;Chakrabarti, AK;Albari?o, CG;Flint, M;Nichol, ST;Spiropoulou, CF;, MBio 2016 ,PubMed ID 27999160 [MYC]
Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer ,Xiao, T;Zhu, JJ;Huang, S;Peng, C;He, S;Du, J;Hong, R;Chen, X;Bode, AM;Jiang, W;Dong, Z;Zheng, D;, Oncogene 2016 ,PubMed ID 27893713 [MYC]
The Role and Regulation of Mitochondrial Dynamics in Cisplatin Resistance in Human Gynecologic Cancer Cells ,Kong, B;, Thesis Jun 2015 [Myc]
Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs ,Donohoe, OH;Henshilwood, K;Way, K;Hakimjavadi, R;Stone, DM;Walls, D;, PLoS ONE May 2015 ,PubMed ID 25928140 [MYC]
Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes ,Lauranzano, E;Pozzi, S;Pasetto, L;Stucchi, R;Massignan, T;Paolella, K;Mombrini, M;Nardo, G;Lunetta, C;Corbo, M;Mora, G;Bendotti, C;Bonetto, V;, Brain Feb 2015 ,PubMed ID 25678563 [Myc]
[IP]
Huntingtin-associated protein 1 interacts with breakpoint cluster region protein to regulate neuronal differentiation ,Huang, PT;Chen, CH;Hsu, IU;Salim, SA;Kao, SH;Cheng, CW;Lai, CH;Lee, CF;Lin, YF;, PLoS ONE Feb 2015 ,PubMed ID 25671650 [Myc]
[IF]
SHP2 Positively Regulates TGF?1-induced Epithelial-mesenchymal Transition Modulated by Its Novel Interacting Protein Hook1 ,Li, S;Wang, L;Zhao, Q;Liu, Y;He, L;Xu, Q;Sun, X;Teng, L;Cheng, H;Ke, Y;, J. Biol. Chem. Oct 2014 ,PubMed ID 25331952 [Myc]
[IP]
p53 is required for cisplatin-induced processing of the mitochondrial fusion protein L-Opa1 that is mediated by the mitochondrial metallopeptidase Oma1 in Gynecologic Cancers ,Kong, B;Wang, Q;Fung, E;Xue, K;Tsang, BK;, J. Biol. Chem. Aug 2014 ,PubMed ID 25112877 [Myc]
[WB]
Holocarboxylase synthetase interacts physically with the nuclear receptor corepressor, histone deacetylase 1, and a novel splicing variant of histone deacteylase 1 to repress repeats ,Liu, D;Zempleni, J;, Biochem. J. May 2014 ,PubMed ID 24840043 [Myc]
[IP]
Novel trafficking-defective HCN4 mutation is associated with early-onset atrial fibrillation ,Macri, V;Mahida, SN;Zhang, ML;Sinner, MF;Dolmatova, EV;Tucker, NR;McLellan, M;Shea, MA;Milan, DJ;Lunetta, KL;Benjamin, EJ;Ellinor, PT;, Heart Rhythm Mar 2014 ,PubMed ID 24607718 [anti-myc]
[IF]
The PTH-Gαs-PKA cascade controls αNAC localization to regulate bone mass ,Pellicelli, M;Miller, JA;Arabian, A;Gauthier, C;Akhouayri, O;Wu, JY;Kronenberg, HM;St-Arnaud, R;, Mol. Cell. Biol., Feb 2014. ,PubMed ID 24550008 [anti-MYC]
[WB]
From Histone Modification to Gene Repression: Epigenetic Regulation by Holocarboxylase Synthetase-containing Repression Complex and FAD-dependent Lysine … ,Liu, D;, Thesis . March 2014 [MYC]
[IP]
Glia maturation factor- mediates neutrophil chemotaxis ,Wulin Aerbajinai, Lunhua Liu, Kyung Chin, Jianqiong Zhu, Carole A. Parent, and Griffin P. Rodgers, J. Leukoc. Biol., Sep 2011; 90: 529 – 538. ,PubMed ID 21653232 [MYC]
[IP]
Mechanism of Down-regulation of RNA Polymerase III-transcribed Non-coding RNA Genes in Macrophages by Leishmania ,Tanu Rana, Smita Misra, Mukul K. Mittal, Anitra L. Farrow, Keith T. Wilson, MacRae F. Linton, Sergio Fazio, Ian M. Willis, and Gautam Chaudhuri, J. Biol. Chem., Feb 2011; 286: 6614 – 6626 ,PubMed ID 21149457 [MYC]
[IP]

ACEAnti-c-Myc Antibody

產(chǎn)品介紹:

c-Myc 蛋白含有 bHLH 結(jié)構(gòu)域,該蛋白在正常機(jī)體發(fā)育和腫瘤發(fā)生過(guò)程中起著非常重要的作用,c-Myc 是一個(gè)重要的轉(zhuǎn)錄因子,其異常表達(dá)能促進(jìn)多種癌癥的的發(fā)生和發(fā)展。將人 p62 c-Myc蛋白的 410-419 這段氨基酸序列(EQKLISEEDL)融合至目的蛋白的 N 端或者是 C 端,可以對(duì)目的蛋白進(jìn)行準(zhǔn)確的檢測(cè)、定位和純化。
抗體來(lái)源:小鼠
交叉反應(yīng):c-Myc 標(biāo)簽融合蛋白
克隆類(lèi)型:?jiǎn)慰寺】贵w
亞型:IgG2A
來(lái)源:293 細(xì)胞表達(dá)的重組抗體
純化方式:rProtein A 親和純化
產(chǎn)品純度:≥95%,SDS-PAGE 檢測(cè)
儲(chǔ)存緩沖液:1×PBS (pH7.4) ,0.02%疊氮鈉,50%甘油
儲(chǔ)存條件:干冰運(yùn)輸,-20℃可保存二年,避免反復(fù)凍融

產(chǎn)品應(yīng)用:

濃度:1 mg/mL
WB=1/2000-1/30000
ELISA=1/5000-1/15000
IP=0.5μg-4μg
IF=1/200

產(chǎn)品信息:

貨號(hào) 名稱(chēng) 規(guī)格
AB001-01 Anti-c-Myc Antibody 100μg
AB001-03 Anti-c-Myc Antibody 1000μg