標(biāo)簽歸檔：antibody

SIGMA A2052-100UL 抗GABA抗體 兔抗

發(fā)表于由

屬性
生物來源
rabbit

質(zhì)量水平
200

偶聯(lián)物
unconjugated

抗體形式
affinity isolated antibody

antibody product type
primary antibodies

克隆
polyclonal

形式
buffered aqueous solution

species reactivity
rat, Drosophila, wide range

包裝
antibody small pack of 25 μL

technique(s)
dot blot: 1:10,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum

運(yùn)輸
dry ice

儲存溫度
20°C

Gene Information

rat … Gabra1(29705)

說明
一般描述
抗GABA抗體是使用GABA-BSA作為兔的免疫原進(jìn)行生產(chǎn)的。通過免疫特異性純化方法從抗血清中分離抗體?？乖禺愋杂H和分離可去除幾乎所有兔血清蛋白，包括不與GABA特異性結(jié)合的免疫球蛋白。
GABA（γ-氨基丁酸）是存在于從原核生物到脊椎動物的多種生物體內(nèi)的多功能分子。它存在于非神經(jīng)結(jié)構(gòu)中，如外周神經(jīng)和內(nèi)分泌系統(tǒng)。[1]谷氨酸脫羧酶（GAD）催化谷氨酸發(fā)生脫羧反應(yīng)，形成GABA。
免疫原
γ-氨基丁酸(GABA)-BSA結(jié)合物。
應(yīng)用
使用兔抗GABA抗體通過免疫熒光法檢測從E19天大鼠胚胎大腦神經(jīng)細(xì)胞中GABA的表達(dá)。用抗GABA抗體和兩種抗GAD抗體進(jìn)行三重IF染色。通過免疫組織化學(xué)檢測方法，分析從各種動物（包括大鼠、小鼠、兔、豚鼠和蜥蜴）大腦皮層分離的細(xì)胞中GABA的表達(dá)。使用以1:1000比例溶于0.01M PBS（pH 7.4）+0.5％triton-x100溶液中的兔抗GABA抗體進(jìn)行IHC分析。
兔抗GABA抗體可用于大鼠海馬組織的電子顯微鏡分析，稀釋度為1:4000。該抗體還被用于果蠅和美洲大蠊腦細(xì)胞的免疫細(xì)胞化學(xué)分析，稀釋度范圍為1:100-1:750。
生化/生理作用
GABA（γ-氨基丁酸）是中樞神經(jīng)系統(tǒng)的主要抑制性神經(jīng)遞質(zhì)，并且是一些外周組織的神經(jīng)調(diào)節(jié)劑。它能夠控制心率、血壓和呼吸過程。GABA通過與GABA/苯二氮受體發(fā)生相互作用并打開氯離子通道而促進(jìn)神經(jīng)元抑制。在斑點(diǎn)雜交印跡試驗(yàn)中，兔抗GABA抗體表現(xiàn)出與GABA和GABA-鑰孔蟲戚血蘭素（KLH）發(fā)生陽性結(jié)合，并與牛血清白蛋白（BSA）發(fā)生陰性結(jié)合。
外形
溶于0.01M磷酸鹽緩沖鹽水，pH 7.4，含有1% BSA和15 mM 疊氮化鈉。
儲存及穩(wěn)定性
若需連續(xù)使用，可在2-8°C下保存最長一個月。若需延長儲存時間，可將溶液分裝并冷凍。不建議反復(fù)凍融。如果長期儲存時出現(xiàn)輕微渾濁，請在使用前通過離心澄清溶液。
免責(zé)聲明
除非我們的產(chǎn)品目錄或產(chǎn)品附帶的其他公司文檔另有說明，否則我們的產(chǎn)品僅供研究使用，不得用于任何其他目的，包括但不限于未經(jīng)授權(quán)的商業(yè)用途、 體外 診斷用途、 離體 或 體內(nèi) 治療用途或任何類型的消費(fèi)或應(yīng)用于人類或動物。

SIGMA MAB1217-I Anti-Fmc-7 (B-Cell Lymphocyte Marker), clone Fmc-7 Antibody

發(fā)表于由

屬性
生物來源
mouse

質(zhì)量水平
100

抗體形式
purified immunoglobulin

antibody product type
primary antibodies

克隆
Fmc-7, monoclonal

species reactivity
human

technique(s)
flow cytometry: suitable
western blot: suitable

同位素/亞型
IgMκ

運(yùn)輸
wet ice

Gene Information

human … MS4A1(931)

說明
一般描述
B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.
免疫原
HRIK [human B-lymphoblastoid cell] corresponding to human Fmc-7 (B-Cell Lymphocyte Marker).
應(yīng)用
Flow Cytometry Analysis: 4.0 μg of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in peripheral blood mononuclear cells.
This Anti-Fmc-7 (B-Cell Lymphocyte Marker) antibody is validated for use in WB, FC for the detection of Fmc-7 (B-Cell Lymphocyte Marker).
質(zhì)量
Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2.0 μg/mL of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in 10 μg of THP-1 cell lysate.
目標(biāo)描述
~105 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Ammonium Sulfate
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing 0.1M Tris, 10mM Glycine, 0.5M NaCl, and 0.1% Sodium Azide.
其他說明
Concentration: Please refer to lot specific datasheet.
免責(zé)聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Anti-Swine IgG (H+L) Antibody（5210-0173）

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KPL Antibodies & Conjugates

Anti-Swine IgG (H+L) Antibody

Anti-Swine IgG (H+L) Antibody

貨號：5210-0173

規(guī)格：1mg

品牌：seracare

Anti-Swine IgG (H+L) Antibody（5210-0173）


Material Number5210-0173
Size1 mg
Inventory StatusIn Stock
UnitEA


    DETAILS
Affinity-purified polyclonal antibody to swine IgG, both heavy and light chains (whole IgG), made in goat. Product is in lyophilized form. Each lot is tested to assure specificity and lot-to-lot consistency using an in-house ELISA assay.

Reference Number: 01-14-06

Mouse monoclonal eGFP antibody, clone OTI5A2小鼠單克隆 eGFP 抗體，克隆 OTI5A2

發(fā)表于由
Product Data
Product Name Mouse monoclonal eGFP antibody, clone OTI5A2
Clone Name OTI5A2 (formerly 5A2)
Applications WB
Target eGFP
Host Mouse
Clonality Monoclonal
Immunogen A full length eGFP tagged recombinant protein expressed in HEK293T cell
Isotype IgG1
Formulation PBS (pH 7.3) containing 1%BSA, 50% glycerol and 0.02% sodium azide
Concentration 0.7mg/ml
Conjugation Unconjugated
Storage Condition Store at -20°C.
Predicted Protein Size 26.9 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Acetylated tau inhibits chaperone-mediated autophagy and promotes tau pathology propagation in mice ,Caballero, B;Bourdenx, M;Luengo, E;Diaz, A;Sohn, PD;Chen, X;Wang, C;Juste, YR;Wegmann, S;Patel, B;Young, ZT;Kuo, SY;Rodriguez-Navarro, JA;Shao, H;Lopez, MG;Karch, CM;Goate, AM;Gestwicki, JE;Hyman, BT;Gan, L;Cuervo, AM;, Nature communications 2021 ,PubMed ID 33854069 [EGFP]
A Small Cysteine-Free Protein Acts as a Novel Regulator of Fungal Insect-Pathogenic Lifecycle and Genomic Expression ,Mou, YN;Fu, B;Ren, K;Ying, SH;Feng, MG;, mSystems 2021 ,PubMed ID 33758028 [EGFP]
Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1 ,Li, X;Li, S;Li, B;Li, Y;Aman, S;Xia, K;Yang, Y;Ahmad, B;Wu, H;, NPJ precision oncology 2021 ,PubMed ID 33742100 [EGFP]
Strategies to prevent cleavage of the linker region between ligand-binding repeats 4 and 5 of the LDL receptor ,Str?m, TB;Bjune, K;da Costa, LT;Leren, TP;, Hum. Mol. Genet. 2019 ,PubMed ID 31332430 [EGFP]
MACROD2 Haploinsufficiency Impairs Catalytic Activity of PARP1 and Promotes Chromosome Instability and Growth of Intestinal Tumors ,Sakthianandeswaren, A;Parsons, M;Mouradov, D;MacKinnon, RN;Catimel, B;Liu, S;Palmieri, M;Love, CG;Jorissen, RN;Li, S;Whitehead, L;Putoczki, TL;Preaudet, A;Tsui, C;Nowell, CJ;Ward, RL;Hawkins, NJ;Desai, J;Gibbs, P;Ernst, M;Street, I;Buchert, M;Sieber, OM;, Cancer Discov 2018 ,PubMed ID 29880585 [GFP]
Antagonizing STAT5B dimerization with an osmium complex ,Liu, LJ;Wang, W;Kang, TS;Liang, JX;Liu, C;Kwong, DW;Wong, VK;Ma, DL;Leung, CH;, Sci Rep 2016 ,PubMed ID 27853239 [EGFP]
N-cadherin coordinates AMP kinase-mediated lung vascular repair ,Jian, MY;Liu, Y;Li, Q;Wolkowicz, P;Alexeyev, M;Zmijewski, J;Creighton, J;, Am. J. Physiol. Lung Cell Mol. Physiol 2016 ,PubMed ID 26545901 [EGFP]
HuR antagonizes the effect of an intronic pyrimidine-rich sequence in regulating WT1+/- KTS isoforms ,Li, H;Hou, S;Hao, T;Azam, S;Liu, C;Shi, L;Lei, H;, RNA biology Dec 2015 ,PubMed ID 26512748 [eGFP]
CD14 dependence of TLR4 endocytosis and TRIF signaling displays ligand specificity and is dissociable in endotoxin tolerance ,Rajaiah, R;Perkins, DJ;Ireland, DD;Vogel, SN;, Proc. Natl. Acad. Sci. U.S.A. Jul 2015 ,PubMed ID 26106158 [eGFP]
Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export ,Haixin Lei, Bo Zhai, Shanye Yin, Steve Gygi, and Robin Reed, Nucleic Acids Res., Dec 2012; 10.1093/nar/gks1314. ,PubMed ID 23275560 [eGFP]

HA mouse monoclonal antibody, clone CB051；HA小鼠單克隆抗體，克隆CB051

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 Product Data
Product Name HA mouse monoclonal antibody, clone CB051
Clone Name CB051
Applications WB
Target HA tag
Host Mouse
Clonality Monoclonal
Immunogen Monoclonal antibody is produced by immunizing mice with a synthetic peptide from influenza hemaglutinin coupled to KLH
Isotype IgG1
Formulation PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 1 mg/ml
Conjugation Unconjugated
Storage Condition Store at -20°C.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
VRK2 is involved in the innate antiviral response by promoting mitostress-induced mtDNA release ,He, WR;Cao, LB;Yang, YL;Hua, D;Hu, MM;Shu, HB;, Cellular & molecular immunology 2021 ,PubMed ID 33785841 [HA]
WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation ,Xiong, Y;Yuan, L;Chen, S;Xu, H;Peng, T;Ju, L;Wang, G;Xiao, Y;Wang, X;, Cell Death Dis 2020 ,PubMed ID 32678075 [HA]
Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis ,Zhong, L;Hu, MM;Bian, LJ;Liu, Y;Chen, Q;Shu, HB;, Cell Discov 2020 ,PubMed ID 32351706 [HA]
Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners ,Gallardo-Vara, E;Ruiz-Llorente, L;Casado-Vela, J;Ruiz-Rodríguez, MJ;López-Andrés, N;Pattnaik, AK;Quintanilla, M;Bernabeu, C;, Cells 2019 ,PubMed ID 31540324 [HA]
Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity ,Hu, MM;He, WR;Gao, P;Yang, Q;He, K;Cao, LB;Li, S;Feng, YQ;Shu, HB;, Cell Res. 2019 ,PubMed ID 30651583 [HA]
The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection ,Hara, H;Seregin, SS;Yang, D;Fukase, K;Chamaillard, M;, Cell 2018 ,PubMed ID 30392956 [HA]
Human Cytomegalovirus Protein UL31 Inhibits DNA Sensing of cGAS to Mediate Immune Evasion ,Huang, ZF;Zou, HM;Liao, BW;Zhang, HY;Yang, Y;Fu, YZ;Wang, SY;Luo, MH;Wang, YY;, Cell Host Microbe 2018 ,PubMed ID 29937271 [HA]
CDK5 Inhibition Resolves PKA/cAMP-Independent Activation of CREB1 Signaling in Glioma Stem Cells ,Mukherjee, S;Tucker-Burden, C;Kaissi, E;Newsam, A;Duggireddy, H;Chau, M;Zhang, C;Diwedi, B;Rupji, M;Seby, S;Kowalski, J;Kong, J;Read, R;Brat, DJ;, Cell Rep 2018 ,PubMed ID 29742423 [HA]
Poly(ADP-ribose) Polymerase 1, PARP1, modifies EZH2 and inhibits EZH2 histone methyltransferase activity after DNA damage ,Caruso, LB;Martin, KA;Lauretti, E;Hulse, M;Siciliano, M;Lupey-Green, LN;Abraham, A;Skorski, T;Tempera, I;, Oncotarget 2018 ,PubMed ID 29535829 [HA]
Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition ,Collart, C;Smith, JC;Zegerman, P;, Dev. Cell 2017 ,PubMed ID 28697335 [HA]
Variants in TRIM22 That Affect NOD2 Signaling Are Associated With Very-Early-Onset Inflammatory Bowel Disease ,Li, Q;Lee, CH;Peters, LA;Mastropaolo, LA;Thoeni, C;Elkadri, A;Schwerd, T;Zhu, J;Zhang, B;Zhao, Y;Hao, K;Dinarzo, A;Hoffman, G;Kidd, BA;Murchie, R;Al Adham, Z;Guo, C;Kotlarz, D;Cutz, E;Walters, TD;Shouval, DS;Curran, M;Dobrin, R;Brodmerkel, C;Snapper, SB;Klein, C;Brumell, JH;Hu, M;Nanan, R;Snanter-Nanan, B;Wong, M;Le Deist, F;Haddad, E;Roifman, CM;Deslandres, C;Griffiths, AM;Gaskin, KJ;Uhlig, HH;Schadt, EE;Muise, AM;, Gastroenterology 2016 ,PubMed ID 26836588 [HA]
EZH1/SUZ12 complex positively regulates the transcription of NF-?B target genes via interaction with UXT ,Su, SK;Li, CY;Lei, PJ;Wang, X;Zhao, QY;Cai, Y;Wang, Z;Li, L;Wu, M;, J. Cell. Sci. Apr 2016 ,PubMed ID 27127229 [HA]
The Key Regulator for Language and Speech Development, FOXP2, is a Novel Substrate for SUMOylation ,Meredith, LJ;Wang, CM;Nascimento, L;Liu, R;Wang, L;Yang, WH;, J. Cell. Biochem. Jul 2015 ,PubMed ID 26212494 [HA]
Recombinant Murine Gamma Herpesvirus 68 Carrying KSHV G Protein-Coupled Receptor Induces Angiogenic Lesions in Mice ,Zhang, J;Zhu, L;Lu, X;Feldman, ER;Keyes, LR;Wang, Y;Fan, H;Feng, H;Xia, Z;Sun, J;Jiang, T;Gao, SJ;Tibbetts, SA;Feng, P;, PLoS Pathog. Jun 2015 ,PubMed ID 26107716 [HA]
TRIM4 modulates type I interferon induction and cellular antiviral response by targeting RIG-I for K63-linked ubiquitination ,Yan, J;Li, Q;Mao, AP;Hu, MM;Shu, HB;, J Mol Cell Biol 154-63 6 2. April 2014 ,PubMed ID 24755855 [anti-HA]

DDK (FLAG) mouse monoclonal antibody, clone OTI11C3DDK（FLAG）小鼠單克隆抗體，克隆OTI11C3

發(fā)表于由
 Product Data
Product Name DDK (FLAG) mouse monoclonal antibody, clone OTI11C3
Clone Name OTI11C3
Applications ICC, IHC, WB
Target DDK (FLAG tag): DYKDDDDK
Host Mouse
Clonality Monoclonal
Immunogen Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Isotype IgG1
Formulation PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 1 mg/ml
Conjugation Unconjugated
Storage Condition Store at -20°C.
Note This anti-DDK (FLAG) antibody recognizes both N-terminus and C-terminus DDK tags. It is recommended for Western blot only.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Targeting codon 158 p53-mutant cancers via the induction of p53 acetylation ,Kong, LR;Ong, RW;Tan, TZ;Mohamed Salleh, NAB;Thangavelu, M;Chan, JV;Koh, LYJ;Periyasamy, G;Lau, JA;Le, TBU;Wang, L;Lee, M;Kannan, S;Verma, CS;Lim, CM;Chng, WJ;Lane, DP;Venkitaraman, A;Hung, HT;Cheok, CF;Goh, BC;, Nat Commun 2020 ,PubMed ID 32350249 [DDK]
Overexpression of Progerin Results in Impaired Proliferation and Invasion of Non-Small Cell Lung Cancer Cells ,Hu, XT;Song, HC;Yu, H;Wu, ZC;Liu, XG;Chen, WC;, Onco Targets Ther 2020 ,PubMed ID 32280239 [DDK]
Serine/threonine protein phosphatase 5 is a potential therapeutic target in cholangiocarcinoma ,Hu, MH;Huang, TT;Chao, TI;Chen, LJ;Chen, YL;Tsai, MH;Liu, CY;Kao, JH;Chen, KF;, Liver Int. 2018 ,PubMed ID 29797403 [DDK]
Unfractionated Heparin Alleviates Human Lung Endothelial Barrier Dysfunction Induced by High Mobility Group Box 1 Through Regulation of P38-GSK3β-Snail Signaling Pathway ,Luan, Z;Hu, B;Wu, L;Jin, S;Ma, X;Zhang, J;Wang, A;, Cell. Physiol. Biochem. 2018 ,PubMed ID 29719300 [DDK]
Inhibition of dihydrotestosterone synthesis in prostate cancer by combined frontdoor and backdoor pathway blockade ,Fiandalo, MV;Stocking, JJ;Pop, EA;Wilton, JH;Mantione, KM;Li, Y;Attwood, KM;Azabdaftari, G;Wu, Y;Watt, DS;Wilson, EM;Mohler, JL;, Oncotarget 2018 ,PubMed ID 29541409 [DDK]
TAOK1 negatively regulates IL-17-mediated signaling and inflammation ,Zhang, Z;Tang, Z;Ma, X;Sun, K;Fan, L;Fang, J;Pan, J;Wang, X;An, H;Zhou, J;, Cell. Mol. Immunol. 2018 ,PubMed ID 29400705 [DDK]
SMIT1 Modifies KCNQ Channel Function and Pharmacology by Physical Interaction with the Pore ,Manville, RW;Neverisky, DL;Abbott, GW;, Biophysical Journal 2017 ,PubMed ID 28793216 [DDK]
Exosomes expressing carbonic anhydrase 9 promote angiogenesis ,Horie, K;Kawakami, K;Fujita, Y;Sugaya, M;Kameyama, K;Mizutani, K;Deguchi, T;Ito, M;, Biochem. Biophys. Res. Commun. 2017 ,PubMed ID 28851650 [DDK]
Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-coding RNA ,Martinez-Moreno, M;O'Shea, TM;Zepecki, JP;Olaru, A;Ness, JK;Langer, R;Tapinos, N;, Cell Rep 2017 ,PubMed ID 28834756 [DDK]
Involvement of p38-βTrCP-Tristetraprolin-TNFα axis in radiation pneumonitis ,Krishnamurthy, PM;Shukla, S;Ray, P;Mehra, R;Nyati, MK;Lawrence, TS;Ray, D;, Oncotarget 2017 ,PubMed ID 28548957 [DDK]
OCT4B1 regulates the cellular stress response of human dental pulp cells with ,Liu, L;Huang, R;Yang, R;Wei, X;, BioMed Research International 2017 ,PubMed ID 28473980 [DDK]
Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer ,Xiao, T;Zhu, JJ;Huang, S;Peng, C;He, S;Du, J;Hong, R;Chen, X;Bode, AM;Jiang, W;Dong, Z;Zheng, D;, Oncogene 2016 ,PubMed ID 27893713 [DDK]